International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 2
First page : (511) Last page : (516)
Article doi: : http://dx.doi.org/10.18782/2320-7051.4050
Cytolocalisation of PDC-109 Protein in Ejaculated Spermatozoa of Buffaloe (Bubalus bubalis)
Sunilkumar Patil1*, K.V. Jamuna1, Lakshminarayana Somashekar2, Sellappan Selvaraju2,
R.V. Prasad3 and Binsila B. Krishnan2
1Department of Veterinary Anatomy,Veterinary College, Bangalore, KVAFSU, Bidar
2Reproductive Physiology Laboratory, Animal Physiology Division, ICAR- NIANP, Adugodi, Bangalore.
3 Vice-chancellor, KVAFSU, Bidar
*Corresponding Author E-mail: drsunilpatil681@gmail.com
Received: 14.06.2017 | Revised: 26.07.2017 | Accepted: 1.08.2017
ABSTRACT
Semen quality evaluation is important for improving fertility of animals especially the dairy cows. The aim of the present study was to localize PDC-109, one of the fertility regulating proteins in buffalo spermatozoa. Indirect immunofluorescence was performed in spermatozoa to localize PDC-109. The semen samples were fixed in paraformaldehyde and the PDC-109 was localized using goat polyclonal primary antibody (1:50 dilution) and chicken anti-goat-FITC as secondary antibody (1:200 dilution). The PDC-109 was localized in the apical portion of the acrosomal membrane and post-acrosome region of the buffalo spermatozoa. Since, PDC-109 was localized in the acrosome membrane, it may bind to the choline phospholipids, and may play an important roles in capacitation and acrosome reaction, motility regulation as well as to form oviduct sperm reservoir before fertilization. PDC-109 also localized in the post acrosomal region indicating that PDC-109 might be involved in the sperm penetration deep into the oocyte cytoplasm. This is the first report localized PDC-109 in buffalo spermatozoa.
Key words: Buffalo, Spermatozoa, Indirect immunofluorescence, PDC-109, Acrosome
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Patil, S., Jamuna, K.V., Somashekar, L., Selvaraju, S., Prasad, R.V. and Krishnan, B.B., Cytolocalisation of PDC-109 Protein in Ejaculated Spermatozoa of Buffaloe (Bubalus bubalis), Int. J. Pure App. Biosci.6(2): 511-516 (2018). doi: http://dx.doi.org/10.18782/2320-7051.4050
