International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 2
First page : (196) Last page : (201)
Article doi: : http://dx.doi.org/10.18782/2320-7051.6374

Assessment of DNA Damage by Alkaline Comet Assay in Occupationally Exposed Welders of Hyderabad, Telangana

Chandana Lakkaraju¹*, Indira Priyadarshini², Sridevi Tirumalaraju², Srinivas Bandaru¹, G. Deepika¹ and Hema Prasad Mundluru¹

¹Institute of Genetics and Hospital for Genetic Diseases, Osmania University,
Begumpet, Hyderabad 500016, India
²Department of Genetics, Bhagwan Mahavir medical research centre, Hyderabad
*Corresponding Author E-mail: chandanalakkaraju@gmail.com
Received: 5.03.2018  |  Revised: 1.04.2018   |  Accepted: 7.04.2018  

 ABSTRACT

Welding  work is one of the pervasive industrial profession  in Hyderabad,Telangana. Pin-sized or no attention is usually focused at occupational health risk by local or state authorities in Hyderabad. The present work was aimed at the assessment  of DNA damage in welding  workers by Alkaline Comet Assay(Single cell gel electrophoresis) in blood lymphocytes. The results revealed that there was  statistically significant  difference between the level of DNA damage in blood lymphocytes of welders  and control group (p < 0.05). In addition, the level of damage to DNA in blood of subjects with long term exposure and old age is of serious concern. There is the need to screen occupational activities that can pose serious health risks. The relative ignorance of the welding  workers  about the health risks they are exposed to as well as the public should be addressed.

Key words:  Welding  workers, Occupational exposure to welding fumes, DNA damage

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Lakkaraju C., Priyadarshini, I., Tirumalaraju, S., Bandaru, S., Deepika, G.  and Mundluru, H.P., Assessment of  DNA  Damage by Alkaline Comet Assay in Occupationally Exposed Welders   of  Hyderabad, Telangana, Int. J. Pure App. Biosci.6(2): 196-201 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6374