International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 2
First page : (995) Last page : (1004)
Article doi: : http://dx.doi.org/10.18782/2320-7051.6400

Molecular Characterisation of Leptospira interrogans, Isolated from Animal Samples

C. Ragunath*, S. Komathi and R. Ragunathan

Department of Biotechnology, Hindusthan College of Arts and Science, Coimbatore, Tamil Nadu
*Corresponding Author E-mail: raguraj2611@gmail.com
Received: 9.03.2018  |  Revised: 16.04.2018   |  Accepted: 20.04.2018  

 

 ABSTRACT

Leptospirosis is a zoonosis of worldwide distribution, endemic mainly in countries with humid subtropical or tropical climates and has epidemic potential. Leptospirosis, caused by Leptospira interrogans, is highly infectious emerging water borne zoonosis of global significance. It is an enigmatic life threatening disease, which results into high morbidity and mortality, particularly in poor resource nations. The molecular identification and differentiation Leptospirosis are important for epidemiological and health surveillance. The severe infection risk to organ failure and internal haemorrhage. In the present study, the animal samples were collected and sub cultured on lactose broth. Further they confirmed on Ellinghausen McCullough Johnsn Harris (EMJH) Medium. The spirillum colony was observed under Compound microscope.  The DNA isolated and amplified with PCR using specific primers [16S RNA] and the characters are confirmed by Random amplified polymorphic DNA & Restriction Fragment length polymorphism technique. And their Multi Drug Resistant pattern was confirmed using commercial antibiotic disc.

Key words: Leptospira interrogans, EMJH, 16S RNA Nested PCR, RAPD, RFLP.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Ragunath, C., Komathi, S. and Ragunathan, R., Molecular Characterisation of Leptospira Interrogans, Isolated from Animal Samples, Int. J. Pure App. Biosci.6(2): 995-1004 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6400