International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 1
First page : (532) Last page : (536)
Article doi: http://dx.doi.org/10.18782/2320-7051.5417
Demonstration of Cytochemical Reaction of Alkaline Phosphatase, Myeloperoxidase and Glycogen in the Milk Polymorphonuclear Cells of Mastitic and Healthy Sheep and Goats
Aazima Shah1*, M.M. Darzi1, Rafia Maqbool2, Bisma Kashani1, Rayeesa Ali1, Shayaib Kamil1, Henna Wani1 and Akeel Bashir1
1Division of Veterinary Pathology, Faculty of Veterinary Science & Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology Of Kashmir (SKUASTK). 190006
2Division of Veterinary Microbiology & Immunology, Faculty of Veterinary Science & Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUASTK). 190006
*Corresponding Author E-mail: aazimashah.shah3@gmail.com
Received: 10.08.2017 | Revised: 19.09.2017 | Accepted: 23.09.2017
ABSTRACT
Small ruminant mastitis is an important disease that affects the productivity of the livestock sector and affects both the quality and quantity of milk. The disease is not always life-threatening but it can cause a remarkable economic loss to the country as lambs and kids remain underfed due to the mastitis in their dams. Leucocytes are recruited from the peripheral blood into the milk, which is enhanced during mastitis and can be used as a diagnostic tool. The enzymatic activities associated with leucocytes in milk (somatic cells, polymorphonuclear cells-PMN cells) also alter during the disease. Therefore, the present study was conducted on the milk samples of mastitic and healthy sheep and goats for demonstration of cytochemical reaction of alkaline phosphatase, myeloperoxidase and glycogen in somatic cells of milk, which is not well documented in small ruminants. A total of fifty samples, each of mastitic and healthy milk of sheep and goats were collected and subjected to cytoenzymatic and cytochemical tests. Alkaline phosphatase was demonstrated as blue colour within the cells, and myeloperoxidase activity as blue coloured granules within the cells. A comparison of cytochemical reaction of myeloperoxidase activity of neutrophils of milk and blood was done and showed that the intensity and the number of neutrophils showing myeloperoxidase activity were less in milk than in blood. Glycogen within the somatic cells was demonstrated as magenta colour and the number of somatic cells was more in the mastitic milk sample of both the species. It was concluded that there is alteration of enzymes in context with increase in somatic cells in the mastitic milk in comparison with normal milk in small ruminants.
Key words: Mastitis, Somatic cells, Cytochemistry, Alkaline phosphatase, Myeloperoxidase, Glycogen.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Shah, A., Darzi, M.M., Maqbool, R., Kashani, B., Ali, R., Kamil, S., Wani, H. and Bashir, A., Demonstration of Cytochemical Reaction of Alkaline Phosphatase, Myeloperoxidase and Glycogen in the Milk Polymorphonuclear Cells of Mastitic and Healthy Sheep and Goats, Int. J. Pure App. Biosci.6(1): 532-536 (2018). doi: http://dx.doi.org/10.18782/2320-7051.5417
