International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 1
First page : (523) Last page : (532)
Article doi: http://dx.doi.org/10.18782/2320-7051.5935
Cellulolytic Enzyme Production by Trichoderma reesei MTCC 164 Using Cattle Dung and Cattle Dung Slurry of Biogas as a Substrate
Ajit Kaur1*, Urmila Gupta Phutela2
*1Department of Biofuels, Centre of Excellence in Farm Machinery, CSIR-CMERI
Gill Road-141006, Ludhiana
2School of Renewable Energy Engineering, Punjab Agricultural University, Ludhiana-141004, Punjab, India
*Corresponding Author E-mail: jtkaur89@gmail.com
Received: 25.10.2017 | Revised: 30.11.2017 | Accepted: 4.12.2017
ABSTRACT
Biological fuel production serves as a sustainable carbon neutral energy source in this era of decreasing fossil fuels. Biogas technology is an important alternative biological fuel. In this context, this paper present the use of cheap and easily available cattle dung and cattle dung slurry for producing cellulolytic enzymes using Trichoderma reesei MTCC 164. Being rich in lignocelluloses, cattle dung and slurry are suitable for cellulolytic enzyme production. In this study, upon optimization for fermentation conditions, it was found that sterilizing the sample by autoclaving do not show significant increase in enzyme production and non-autoclaved samples showed better enzyme production. For cattle dung, concentration of 75%, 10⁶ spores/ml Cbase (185.81U/ml), Incubation period of 15 days showed higher activities i.e. 115.9U/ml Cbase, .While in case of digested biogas slurry, all parameters were more at 50% slurry concentration (i.e. 23.07U/ml Cbase) 106 spores/ml Cbase (17.79 U/ml), Incubation period of 15 days. But the incubation period for maximum enzyme production varies with the type of enzymes and their isozymes. Out of all the three components of cellulase complex produced by Trichoderma reesei MTCC 164, cellobiase was produced to the maximum, followed by Fpase and CMCase both in cattle dung and digested biogas slurry. These enzymes can further be purified for commercial purpose.
Key words: Trichoderma reesei, Biogas, Cellulolytic Enzymes, Cattle Dung, Cattle Dung Slurry.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Kaur, A, Phutela, U.G., Cellulolytic Enzyme Production by Trichoderma reesei MTCC 164 Using Cattle Dung and Cattle Dung Slurry of Biogas as a Substrate, Int. J. Pure App. Biosci.6(1): 523-531 (2018). doi: http://dx.doi.org/10.18782/2320-7051.5935
