International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 1
First page : (1721) Last page : (1727)
Article doi: http://dx.doi.org/10.18782/2320-7051.8248
Assessment of Agronomic and Morphological characters of Somaclonal Variation in Regenerated Pearl Millet Plants
P.H. Thejaswini1, H. Shekar Shetty2 and H.B. Mahesha3*
1Department of Biotechnology, Maharani’s Science College for Women, Mysore-570 005
2Department of Biotechnology, Manasagangotri, University of Mysore, Mysore - 570006
3Department of Sericulture, Yuvaraja’s College, University of Mysore, Mysore - 570005
*Corresponding Author E-mail: hbmseri@yahoo.com
Received: 5.01.2018 | Revised: 12.02.2018 | Accepted: 17.02.2018
ABSTRACT
Somaclones, regenerated from Pearl millet cultivar 5141B, were evaluated for various agronomic and morphological characters. Calli were initiated from seeds on modified Murashige and Skoog’s medium containing 5 mg L-1 of 2, 4-D and regenerated on media supplemented with BAP (3ppm) and activated charcoal (0.3%w/v). Percentage of callus induction and embryonic calli were recorded. Calli with shoot were transferred to a hormone-free half-strength MS medium. The seedlings with well-developed roots were transplanted into pots containing a substrate and were acclimatized. After acclimation, plantlets were transplanted to field conditions. Most of the regenerated plants were phenotypically normal. However, some plantlets were albino, sterile, dwarf, with deformed leaf or with abnormalities in spike morphology. Variation was recorded in the R1 generation. Comparison were made between the somaclones and their parents (non-tissue-cultured plants) for plant height, days of flowering, total number of tillers-basal and nodal, number of fertile tillers and panicle length per plant. Comparison for agronomic and morphological characters between the parents and somaclones and among the somaclones revealed significant differences. The parameters of agronomic importance in somaclones can be further tested for heritability which would be of great importance in reviving the pearl millet cultivars withdrawn from cultivation.
Key words: Tissue culture, Somaclone, Pearl Millet, Plant regeneration, Agronomic characters.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Thejaswini, P.H., Shetty, H.S., & Mahesha, H.B., Assessment of Agronomic and Morphological characters of Somaclonal Variation in Regenerated Pearl Millet Plants, Int. J. Pure App. Biosci.6(1): 1721-1727 (2018). doi: http://dx.doi.org/10.18782/2320-7051.8248
