International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 1
First page : (1340) Last page : (1350)
Article doi: http://dx.doi.org/10.18782/2320-7051.6291
Biosynthesis of Gold Nano Particles Using Sargassum Myriocystum and Evaluation of their Antibacterial Activity
A. Mohamed Ismail*, H. Sheik Jahabar Ali and M. Parthasarathy
Biotechnology Research Laboratary, Department of Biotechnology,
Edayathangudy. G. S. Pillay Arts & Science College, Nagapattinam, Tamil Nadu, India
*Corresponding Author E-mail: biotechismail@gmail.com
Received: 5.01.2018 | Revised: 11.02.2018 | Accepted: 16.02.2018
ABSTRACT
The synthesis of gold nanoparticles (Au) using Sargassum myriocystum (powder or extract) is demonstrated here. The rapid formation of stable Au nanoparticles has been found using S. myriocystum extract in aqueous medium at normal atmospheric condition. Scanning electron microscopy (SEM) analysis revealed that the the size (diameter) of the synthesized gold nanoparticles lie within 40-85 nm and the average size of the nanoparticles is ~ 60 nm. Fourier transform infrared spectroscopy (FTIR) showed Representative spectra of obtained nanoparticles manifests absorption peaks located at about 1022.11, 1156.16 and 1550.68 cm-1 in the region 500–1600 cm−1. The nanoparticles were also evaluated for their antibacterial activities using the agar diffusion method. Muller–Hinton agar plates were prepared. The inoculum suspension of (Pseudomonas, K. oxyctoca, E. facalis, K. pneumonia, V.parahaemolyticus, V. cholera, E. coli, S. typi, S. paratypi, P. vulgaris) were swabbed uniformly in different plates. The results were confirmed that gold nanoparticle has most of its activity against gram negative organism. In XRD analysis the bottom area of the peak is broad. The XRD patterns thus clearly showed that the gold nanoparticles formed by the bio reduction of AuCl4− ions using S. myriocystum.
Key words: S. myriocystum, gold nanoparticles, SEM, FTIR, XRD, Antibacterial activity, Gram (+) ve and (-) ve Bacteria.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Ismail, A.M., Ali, H.S.J. and Parthasarathy, M., Biosynthesis of Gold Nano Particles Using Sargassum Myriocystumand Evaluation of their Antibacterial Activity, Int. J. Pure App. Biosci.6(1): 1340-1350 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6291
