International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 1
First page : (1170) Last page : (1173)
Article doi: http://dx.doi.org/10.18782/2320-7051.5751

Graft Transmission of Brinjal Little Leaf Disease Caused by Candidatus Phytoplasma Trifolii

Rathnamma* and M. S. Patil
Department of Plant Pathology, University of Agricultural Sciences, Krishinagar, UAS
Dharwad-580005, Karnataka, India
*Corresponding Author E-mail: ratnabergi.c@gmail.com
Received: 22.09.2017  |  Revised: 28.10.2017   |  Accepted: 4.11.2017  

 ABSTRACT

Brinjal little leaf disease is caused by Candidatus Phytoplasma trifolii. The disease is transmitted mainly by leaf hoppers and also transmitted by graft. The little leaf Infected plants of brinjal were used as donor or source plants for grafting.  Phytoplasma was transmitted by wedge grafting from donor little leaf infected brinjal to receptor healthy brinjal plants and developed little leaf symptoms within 20-25 days after grafting.  There is no little leaf symptoms development in graft failed plants and control plant that is healthy brinjal scion grafted to healthy brinjal rootstock. All the plants (graft successed and contol palnts) samples were subjected to PCR using primers P1/P7 universal primers specific to 16S rRNA gene of phytoplasmas. Which resulted in expected amplicons of size ~1·8 kb observed only in case of graft successes brinjal plants whereas no amplification in   healthy control plant.

Key words: Brinjal, Phytoplasmas, Virescence, Phyllody, Sterility

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Rathnamma and Patil, M.S., Graft Transmission of Brinjal Little Leaf Disease Caused by Candidatus Phytoplasma Trifolii, Int. J. Pure App. Biosci.6(1): 1170-1173 (2018). doi: http://dx.doi.org/10.18782/2320-7051.5751