International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 1
First page : (713) Last page : (720)
Article doi: http://dx.doi.org/10.18782/2320-7051.6154

Isolation and Characterisation of Cholesterol Biodegrading Bacteria from Industrial Waste, Partial Purification of Cholesterol Oxidase, its Bioreactor and Application Studies

Athul Sanjeev*, Kavyamol K. Nair, Nija R. J., Arya T. Dharan, Nahida Nazeer,
Ananthavishnu S. G. and P. Roopa
Department of Biotechnology and Biochemical Engineering, Mohandas College
of Engineering and Technology, Thiruvananthapuram, kerala, India
*Corresponding Author E-mail: drathulsanjeevtvm@gmail.com
Received: 7.01.2018  |  Revised: 10.02.2018   |  Accepted: 15.02.2018  

 ABSTRACT

Cholesterol oxidase (CHO) is a monomeric bi-functional FAD-containing (flavoenzyme) enzyme belongs to the family of oxidoreductases those catalyses the oxidation of cholesterol into 4-cholesten-3-one. Cholesterol oxidase has various clinical and industrial applications. . Recently, microbial cholesterol oxidase have received a great attention for their wide usage in medicine. The present study aims at isolation and characterization of microorganisms which possess ability to produce CHO when grown in a medium rich in cholesterol. The presence of CHO producing microorganism was confirmed by CHO indicator plates. Biochemical tests and molecular characterization (i.e.) 16s rRNA sequencing was performed to identify the strain as Bacillus sonorensis. Optimum production was observed at 35°C and pH 7.0 on cholesterol minimal medium. The product obtained during fermentation is partially purified by ammonium sulphate precipitation and Dialysis. The purified cholesterol oxidase enzyme was electrophoresised and Molecular weight of this enzyme was estimated at 55 kDa. Antimicrobial activity of purified enzyme was tested. Also the effect of the enzyme on serum cholesterol was studied.

Key words: Bacillus sonorensis, Cholesterol oxidase, Cholesterol, 4-cholesten-3-one, CHO indicator plates.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Sanjeev, A., Nair, K.K., Nija, R.J., Dharan, A.T., Nazeer, N., Ananthavishnu, S.G. and Roopa, P., Isolation and Characterisation of Cholesterol Biodegrading Bacteria from Industrial Waste, Partial Purification of Cholesterol Oxidase, its Bioreactor and Application Studies, Int. J. Pure App. Biosci.6(1): 713-720 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6154