International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 1
First page : (1) Last page : (11)
Article doi: http://dx.doi.org/10.18782/2320-7051.6137
Biological Control of Bacterial Leaf Spot Disease of Papaya (Carica papaya) through Antagonistic Approaches using Medicinal Plants Extracts and Soil Bacteria
Md. Firose Hossain1, SM Zia Hasan1, Zannati Ferdous Zaoti1, Habiba1,
Md. Faruk Hasan1, Md. Asadul Islam1, and Biswanath Sikdar2*
1Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Sciences,
University of Rajshahi, Rajshahi-6205, Bangladesh
2Professor Joarder DNA and Chromosome Research Lab., Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh
*Corresponding Author E-mail: sikdar2014@gmail.com
Received: 24.12.2017 | Revised: 22.01.2018 | Accepted: 27.01.2018
ABSTRACT
The present investigation was carried out to isolate the causal agent of bacterial leaf spot disease of papaya and evaluation of its biological control through different extracts from selected medicinal plants and antagonistic microorganisms. The causal organism was isolated on nutrient agar medium at 370C for incubation period of 16 hours. Molecular detection of the isolated bacteria confirmed by PCR using specific primers 27F (5′-AGAGTTTGATCCTGGCTC-3′) and 1391R (5′-GACGGCGGTGTGTRCA-3′) which amplified approximately 1600 bp DNA fragment of the isolated bacteria. Four different types of solvents Methanol, Ethanol, Acetone, Petroleum ether were used to prepare the extract from Lantana camara, Pongamia pinnata, Rauwolfia serpentina, Cymbopogon citrates, Tagetes erecta. Among them the ethanol extract of Lantana camara showed highest 13.5 mm zone of inhibition at 60µg/ml concentration and methanol extract showed 17mm zone of inhibition at 100µg/ml concentrations respectively against isolated bacteria. Antagonistic soil bacteria were isolated from different soil samples from Rajshahi University premises. Isolate 1 and 2 of soil bacteria showed highest 12±0.2 mm inhibition zone at 40µl/disc concentration in disc diffusion method and isolate 3 of soil bacteria showed 16±0.5 mm inhibition zone against bacterial leaf spot disease causing pathogen at 50 µl/well concentrations in well diffusion method. This study will be helpful for effective biological control of bacterial leaf spot disease of papaya causing less harm to the environment compare to the traditionally available chemical control.
Key words: Papaya, Bacterial leaf spot, Biological control, Antagonistic, Pseudomonas carica papayaFull Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Hossain, Md. F., Hasan, S.M.Z., Zaoti, Z.F., Habiba, Hasan Md. F., Islam Md. A., and Sikdar B., Biological Control of Bacterial Leaf Spot Disease of Papaya (Carica papaya) through Antagonistic Approaches using Medicinal Plants Extracts and Soil Bacteria, Int. J. Pure App. Biosci.6(1): 1-11 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6137
