International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 6
First page : (428) Last page : (436)
Article doi: http://dx.doi.org/10.18782/2320-7051.5028

Characterization and Identification of Dolichos Bean (Lablab purpureus L. sweet) Recombinant Inbred Lines (RIL) with High Pod Yield and High Pod Fragrance

Uday Kumar H. R.¹*, Byre Gowda M.², Ramesh S.¹ and Vasundhara M.^3
1Department of Genetics and Plant Breeding, University of Agricultural Sciences (UAS), Bengaluru, Karnataka, India
²All India Coordinated Research Project on Pigeonpea, UAS, Bengaluru, Karnataka, India
³Department of Horticulture, UAS, Bengaluru, Karnataka, India
*Corresponding Author E-mail: udayhr7096@gmail.com
Received: 21.06.2017  |  Revised: 1.08.2017   |  Accepted: 4.08.2017  

 ABSTRACT

Pod fragrance in dolichos bean is being routinely assessed by organoleptic (smelling) means. Phenotyping pod fragrance by organoleptic means is highly subjective. The relativity and subjectivity associated with organoleptic means of phenotyping pod fragrance could be overcome by objective means, that is by analyzing and quantifying trans 2-Dodecenoic acid and trans 2-Tetradecenoic acids, the key fatty acids (FA) in the pod exudates of dolichos bean. An attempt was made to characterize recombinant inbred lines (RIL) population derived from two connected crosses for pod fragrance by quantifying the two key FA in pod exudates and to explore correspondence between organoleptic and objective means of phenotyping pod fragrance. The variability among RIL was substantial for both the FA as indicated by the estimates of absolute and standardized range and PCV. The positively skewed platykurtic distribution of RIL population for both the key FA indicated that the pod fragrance is controlled by fewer genes with complementary epistasis. The concentration of the two FA in pod exudates of the best 10 RILs of both the populations were higher than that of the mean by at least 60 per cent. The RIL 3-3 was one of the best 10 RIL with respect to concentration of trans 2-Tetradecenoic acid and promising for fresh pods plant-1, fresh pod yield plant-1, and fresh seed yield plant-1. The significance of mean concentration of the two key FA of pod exudates of RIL classified under high, medium and low pod fragrance groups based on organoleptic means by, ‘F’ test indicated good correspondence between objective and organoleptic means and reliability of screening RIL population by organoleptic means.

Key words: Fatty acids, Pod fragrance, Trans-2-dodecenoic acid, Trans-2-tetradecenoic acid

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Uday Kumar, H.R., Gowda, B.M., Ramesh, S. and Vasundhara, M., Characterization and Identification of Dolichos Bean (Lablab purpureus L. sweet) Recombinant Inbred Lines (RIL) with High Pod Yield and High Pod Fragrance, Int. J. Pure App. Biosci.5(6): 428-436 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5028