International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 6
First page : (312) Last page : (317)
Article doi: http://dx.doi.org/10.18782/2320-7051.5783
Conservation of an Endangered Medicinal Plant (Taxus baccata L. Subsp. Wallichiana) of Uttarakhand Himalaya through Embryo Culture
Sobha1* and J. S. Chauhan2
1Corresponding author and Research Scholar Department of Seed Science and Technology, School of Agriculture & Allied Sciences, HNB Garhwal University (A Central University),
Srinagar Garhwal-246174, Uttarakhand, India
2Professor Department of Seed Science and Technology, School of Agriculture & Allied Sciences, HNB Garhwal University (A Central University), Srinagar Garhwal-246174, Uttarakhand, India
*Corresponding Author E-mail: bishtsobha0519@gmail.com
Received: 25.09.2017 | Revised: 29.10.2017 | Accepted: 3.11.2017
ABSTRACT
The objective of present investigates was to develop seedling emergence and seed dormancy breaking techniques of Taxus baccata, an important medicinal plant which seeds have proved difficult to germinate. Seed germination has been studied from many years to develop a procedure to obtain seedling from those seeds which is inhibited due to dormancy. Propagation through seed is slow and cannot respond to the growing demand of planting material for extraction of Taxol (an anticancer drug). Seedlings of Taxus baccata were obtained by embryo culture to overcome the lengthy phase of dormancy in seeds. The de-pulped seeds were dried under controlled laboratory conditions. Embryos isolated from the 100% sterile seeds are used for embryo culture; absorbent activated charcoal 5gm/L shows better result than without absorbent in embryo germination. The best embryo development was seen in 1000 ppm pretreatment gibbrellin+ Murashige and Skoog media + activated charcoal 5gm/L that is 30%. Murashige and Skoog media + activated charcoal 5 gm/L treatment had the next best treatment for embryo germination that is 20%. Storage times cannot affect the rate of seed dormancy.
Key word: Conservation, Taxus baccata, Dormancy, Taxol
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Sobha and Chauhan, J.S., Conservation of an Endangered Medicinal Plant (Taxus baccata L. Subsp. Wallichiana) of Uttarakhand Himalaya through Embryo Culture, Int. J. Pure App. Biosci.5(6): 312-317 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5783
