International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 6
First page : (1608) Last page : (1615)
Article doi: http://dx.doi.org/10.18782/2320-7051.2848
Diagnostic Set of SSR Markers for Molecular Fingerprinting of Parental Lines and Hybrid Purity Testing in Castor (Ricinus communis L.)
Usha Kiran B.1*, Shivakula Sekara Paandian2 and Lavanya C.1
1ICAR-Indian Institute of Oilseeds Research (ICAR-IIOR), Rajendranagar, Hyderabad, Telangana State, India
2Student, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
*Corresponding Author E-mail: ushakiran.b@icar.gov.in
Received: 13.04.2017 | Revised: 21.05.2017 | Accepted: 25.05.2017
ABSTRACT
Microsatellite markers were used for molecular fingerprinting of varieties, parental lines of hybrids released from IIOR and testing the genetic purity of hybrid seed lot of castor. The DNA markers will allow accurate, speedy variety identification and assessing genetic purity of hybrids compared to grow-out test. In this study 110 simple sequence repeats (SSR) markers were employed for fingerprinting 2 popular castor varieties, 2 hybrids and their parental lines. 15 unique alleles were identified for 6 parental lines and a total of 132 alleles was observed with 56 polymorphic markers. The analysis of plant-to plant variation within the parental lines of the hybrid DCH177 and DCH519, using informative markers indicated residual heterozygosity at two marker loci. This highlights the practical utility of SSR markers in maintaining the genetic purity of the parental lines and assessing the genetic purity of the castor hybrids.
Key words: Castor, Fingerprinting, Hybrid purity, Parental lines, SSR markers
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Kiran, B. U., Paandian, S.S. and Lavanya, C., Diagnostic Set of SSR Markers for Molecular Fingerprinting of Parental Lines and Hybrid Purity Testing in Castor (Ricinus communis L.), Int. J. Pure App. Biosci.5(6): 1608-1615 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2848
