International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 6
First page : (1246) Last page : (1250)
Article doi: http://dx.doi.org/10.18782/2320-7051.5226
Deciphering the Mechanism of Mycoparasitism of Sclerotinia sclerotiorum by Trichoderma spp.
Gopi Kishan1*, Manish Kumar2, Rahul Tiwari3 and Pratibha Sharma4
Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi-110012
*Corresponding Author E-mail: gopik0956@gmail.com
Received: 15.07.2017 | Revised: 22.08.2017 | Accepted: 26.08.2017
ABSTRACT
White rot of mustard caused by Sclerotinia sclerotiorum is an important disease and rising to an outbreak level in recent past. Trichoderma is a biocontrol agent which controls the pathogens by various mechanisms viz. mycoparasitism, antibiosis, competition, systemic induced resistance etc., of which hyper-parasitism is the most important mechanism employed by Trichoderma against several plant pathogens. Studies were conducted to decipher the mechanism of mycoparasitism of S. sclerotiorum by Trichoderma spp. A total of eight different species of Trichoderma viz. T. harzianum (Th3), T. harzianum (Th5), T. viride, T. virens, T. asperellum, T. longibrachiatum, T. koningi, T. atroviride were tested against four S. sclerotiorum isolates. Dual culture results revealed that T. viride and two isolates of Trichoderma harzianum Th3 and Th5 were found to be more effective against S. sclerotiorum by inhibiting 71.94, 80.23 and 71.99 per cent mycelial growth respectively. Scanning electron microscopy results shows that T. harzianum (Th3) and T. viride mycoparasitize the S. sclerotiorum by attaching and coiling around by formation of either hyphal coils or hooks and finally degrading the hyphae and sclerotia of pathogen.
Key words: Mycoparasitism, Sclerotinia sclerotiorum, Trichoderma spp., Scanning electron microscopy
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Kishan, G., Kumar, M., Tiwari, R. and Sharma, P., Deciphering the Mechanism of Mycoparasitism of Sclerotinia sclerotiorum by Trichoderma spp., Int. J. Pure App. Biosci.5(6): 1246-1250 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5226
