International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 6
First page : (1113) Last page : (1121)
Article doi: http://dx.doi.org/10.18782/2320-7051.6033
Cloning of HVA22 Homolog from Aloe vera and Preliminary Study of Transgenic Plant Development
Kadirvelu Sharon1 and Sharma Suvarna*
1Department of Biotechnology, *Department of Life Sciences,
Kishinchand Chellaram College, D.W. Road, Churchgate, Mumbai-20
*Corresponding Author E-mail: suvarsharma@rediffmail.com
Received: 23.11.2017 | Revised: 21.12.2017 | Accepted: 24.12.2017
ABSTRACT
Aloe vera (L.) (Aloe barbadensis Miller) is a perennial succulent xerophyte, which develops water storage tissue in the leaves to survive in dry environmental conditions. Few Genes widely known for stress tolerance were isolated from Aloe vera genome and shown to have expressed as well like, DREB1. HVA22 is a stress inducible gene isolated from Hordeum vulgare aleurone layer. Homologs of HVA22 have been found in 354 plant species. The expression of HVA22 in vegetative tissues can be induced by ABA and environmental stresses, such as cold and drought. Aloe vera is known to survive without water for long duration of time. This study aimed at isolating stress inducible genes from Aloe vera. Aloe vera plantlets subjected to ABA were used to isolate cDNA pool. HVA22 homolog was obtained using RT- PCR which was verified by sequencing and bioinformatics. It was inserted into pCAMBIA 2300 and was cloned into Agrobacterium tumefaciens MTCC 431. Agrobacterium mediated gene transfer was performed in Ocimum basilicum, where integration of the transgene was shown upto the genomic level.
Key words: ABA, pCAMBIA 2300, Stress- inducible gene, Ocimum basilicum
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Sharon, K. and Suvarna, S., Cloning of HVA22 Homolog from Aloe vera and Preliminary Study of Transgenic Plant Development, Int. J. Pure App. Biosci.5(6): 1113-1121 (2017). doi: http://dx.doi.org/10.18782/2320-7051.6033
