International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 6
First page : (1024) Last page : (1031)
Article doi: http://dx.doi.org/10.18782/2320-7051.5892
Morphological and Molecular Variability of Lasiodiplodia theobromae Causing Stem End Rot of Mango in Tamil Nadu, India
K. Sathya*, S. Parthasarathy, G. Thiribhuvanamala and K. Prabakar
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore- 641003
*Corresponding Author E-mail: sathya.pathology@gmail.com
Received: 11.10.2017 | Revised: 15.11.2017 | Accepted: 19.11.2017
ABSTRACT
Sixteen isolates of Lasiodiplodia theobromae were collected from mango varieties grown in various regions of Tamil Nadu, India. The results revealed that most of the L. theobromae isolates exhibited significant differences in morphology, colour and spore size. However, all 16 isolates took 3–4 days invariably to cover the 90 mm Petri plates. Among the 16 isolates, the isolates LT 14 from Neelum produced abundant pycnidia and identified as a virulent isolate. These isolates were also analysed through Random amplified polymorphic DNA (RAPD) markers for genetic diversity. Polymerase chain reaction amplification of total genomic DNA of the 16 isolates of L. theobromae with 21 random primers generated numerous polymorphisms among the isolates, while many of the intense bands were shared among majority of the isolates. Cluster analysis also indicated a high degree of genetic variability within L. theobromae isolates from different banana cultivars. The 16 isolates were separated into two clusters namely A and B. The maximum similarity index of 85.40% was recorded between the isolates LT4 and LT5. The least similarity index of 41.60% was recorded with the isolates LT5 and LT7. Within the species of L. theobromae the genetic variability was high and it underlines and validates existence of significant intra-specific diversity in isolates of L. theobromae infecting different mango varieties
Key words: Mango; Stem end rot; Lasiodiplodia theobromae; RAPD analysis
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Sathya, K., Parthasarathy, S., Thiribhuvanamala, G. and Prabakar, K., Morphological and Molecular Variability of Lasiodiplodia theobromae Causing Stem End Rot of Mango in Tamil Nadu, India, Int. J. Pure App. Biosci.5(6): 1024-1031 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5892
