International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 6
First page : (926) Last page : (937)
Article doi: http://dx.doi.org/10.18782/2320-7051.2788
Identification of Efficient Primers for Developing DNA Finger Printing Pattern of Rice Cultivars of West Bengal
P. Panigrahi1, K. K. Panigrahi2* and R. Sadhukhan3
1Department of Horticulture, Govt. of Odisha, India
2College of Agriculture, Chiplima, OUAT, Odisha, India
3Department of Genetics and Plant Breeding, BCKV, West Bengal, India
*Corresponding Author E-mail: kaushikpbg@gmail.com
Received: 2.04.2017 | Revised: 30.05.2017 | Accepted: 4.06.2017
ABSTRACT
In this study 18 rice cultivars of West Bengal were subjected to SSR assay to identify the efficient primers for developing DNA fingerprinting. Five SSR primers of rice (RM206, RM219, RM251, RM231: 27.77% each and RM253: 22.22%) revealed a considerable DNA polymorphism among the selected cultivars of West Bengal considered for the study. The SSR primer RM219 of rice had showed highest discriminating power (0.817) followed by RM231 (0.797) then RM251 (0.758), RM206 (0.719), RM265 (0.679), RM3 (0.542), RM253 (0.471), RM236 (0.366) respectively and were produced polymorphic banding pattern, where as RM190 and RM349 showed monomorphic banding pattern. The highest PIC value had showed by RM265 (0.427), then followed by RM231 (0.376), RM251 (0.357), RM3 (0.341), RM206 (0.339), RM219 (0.308), RM236 (0.230) and RM253 (0.222) respectively. A combination of SSR primers RM219, RM251, RM231 distinguished the cultivar Gontra bhog and Gontra Bidhan-1 from all other cultivars used in the study by generating amplicon of 200, 150, 180 bp and 200, 150, 194 bp fragments respectively. Similarly a combination of RM219, RM206, RM231 distinguished the cultivar CR-1018 and Gontra Bidhan-4 from all other cultivars used in the study by generating amplicon of 210, 150, 180 bp and 200, 150, 200 bp fragments respectively. A combination of RM206, RM231 for IR36; RM206, RM219 for Gontra Bidhan-3 and IR64; RM206, RM231 for MTU-1001 and Lalat; RM251, RM219 for Satabadi, Ratna, and Gobindabhog; were distinguished from all other cultivars used in the study.
Key words: Variability, Primers, SSR, Rice, Combinations.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Panigrahi, P., Panigrahi, K.K. and Sadhukhan, R., Identification of Efficient Primers for Developing DNA Finger Printing Pattern of Rice Cultivars of West Bengal, Int. J. Pure App. Biosci.5(6): 926-937 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2788
