International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 6
First page : (762) Last page : (764)
Article doi: http://dx.doi.org/10.18782/2320-7051.6116

Detection of Phytoplasma Diseases in Sesamum through PCR

Karra Ravindar*
Department of Biotechnology, Kakatiya University, Warangal, Telangana
*Corresponding Author E-mail: rawinder.k@gmail.com
Received: 21.11.2017  |  Revised: 18.12.2017   |  Accepted: 24.12.2017  

 ABSTRACT

Phyllody is a destructive disease of sesamum, Phyllody, Floral virescence and bushy appearance are the most common symptoms produced by phytoplama. Phytoplamal infection from  sesamum was detected by PCR technique. In the present investigation amplified product of expected size is 1.2 kb from phytoplasma infected samples by PCR by using primer pair with R16F2/R16R2 for specific for the 16S rDNA sequences of phytoplasma. The PCR based techniques are very much useful for eradication and  produce pathogen free planting material of sesamum.

Key words: rDNA, PCR based techniques, Sesamum, Phyllody

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Ravindar, K., Detection of Phytoplasma Diseases in Sesamum through PCR, Int. J. Pure App. Biosci.5(6): 762-764 (2017). doi: http://dx.doi.org/10.18782/2320-7051.6116