International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 6
First page : (716) Last page : (724)
Article doi: http://dx.doi.org/10.18782/2320-7051.2824
Morphological and Molecular Characterization of Oyster Mushrooms of Kerala
Krishnapriya, P. J.1*, Geetha, D.2 and Priya, R. U.3
1,3PhD. Scholar, 2Professor,
Department of Plant Pathology, College of Agriculture, Vellayani, Kerala, India
*Corresponding Author E-mail: krishnasaketham@gmail.com
Received: 10.04.2017 | Revised: 26.05.2017 | Accepted: 1.06.2017
ABSTRACT
Pleurotus mushroom, generally referred as the ‘oyster mushroom’is the well appreciated one in Kerala, for its broad adaptability under diverse agro-climatic conditions. There are 15 to 20 species of oyster mushrooms, available from Kerala, of which the common ones are Pleurotus eous and Pleurotus florida. P. eous has soft, pink coloured sporophores. P. florida has decurrent, pure white and fleshy sporophores. New additions to the Pleurotus family, are Pleurotus opuntiae and Pleurotus cystidiosus. Sporocarps of P. opuntiae are stark white, delicately fringed at the edges, borne on short stalks. P. cystidiosus hasbigger size, thick, fleshy, buff coloured and less spore forming sporocarps. The oyster mushrooms were isolated from the natural locations of Kerala and brought in to pure cultures. P. eous, P. florida, P. opuntiae and P. cystidiosus took 9.0, 7.3, 7.0 and 24.7 days for completing the growth in petriplates, when potato dextrose peptone agar was used for culturing. The culture of P. cystidiosus is characteristic, due to the occurrence of black coremial structures, indicating its anamorph stage (Antromycopsis broussonetiae). The morphological identities of the species were confirmed by ITS sequencing and registered at Genbank database (accession numbers: KY214257, KY214256, KY214255, KY214254). A phylogenetic tree was also constructed using Clustal W software, which grouped the four oyster species to three clusters. Here, P. florida and P. eous was found in the same cluster, indicating its common evolutionary lineage.
Keywords: Oyster mushrooms, Morphological, Cultural, ITS sequencing, Phylogenetic analysis
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Krishnapriya, P. J., Geetha, D. and Priya, R.U., Morphological and Molecular Characterization of Oyster Mushrooms of Kerala, Int. J. Pure App. Biosci.5(6): 716-724 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2824
