International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 6
First page : (663) Last page : (673)
Article doi: http://dx.doi.org/10.18782/2320-7051.3091
Detection of Toxigenic and Atoxigenic Strains of Aspergillus flavus in Telangana and Andhra Pradesh
M. Ravi Teja1,2*, K. Vijay Krishna Kumar1, P. Srilakshmi1, H. Sudini1, P. Kishore Varma2and
S. R. Koteswara Rao2
1International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Telangana, India
2College of Agriculture, Professor JayaShankar Telangana State Agricultural University Rajendranagar, Hyderabad-500030, Telanagana, India
*Corresponding Author E-mail: princekuddu61@gmail.com
Received: 5.06.2017 | Revised: 3.07.2017 | Accepted: 7.07.2017
ABSTRACT
In groundnut Aspergillus flavus causes aflatoxin contamination which is a qualitative problem occurring at both pre-and post-harvest stages. These aflatoxins have carcinogenic, hepatotoxic, teratogenic and immuno-suppressive effects. The A. flavus strains which produces aflatoxins are called as toxigenic and which do not produce toxins are called as atoxigenic starins. To detect the toxigenic and atoxigenic starins of A. flavus from Telangana and Andhra Pradesh (AP), pod samples were collected from eight selected oil mills/traders’ in Mahaboobnagar, Rangareddy, Nizamabad, Karimnagar (Telangana); and Anantapur (AP) districts. A total of 24 A. flavus cultures were isolated from the collected pod samples. These isolates were identified as toxigenic/atoxigenic using cultural detection methods on Yeast extract sucrose (YES) media and coconut agar medium (CAM). Based on cultural methods, it was confirmed that there were18 toxigenic, five atoxigenic and one false positive/negative strain out of the 24 A. flavus isolates obtained from surveyed oil mills. Atoxigenic strains were obtained from Karimnagar and Nizamabad districts of Telangana.
Key words: Groundnut, Aflatoxins, Aspergillus flavus, Coconut agar medium.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Teja, M.R., Kumar, K.V.K., Srilakshmi, P., Sudini, H., Varma, P.K. and Rao, S.R.K., Detection of Toxigenic and Atoxigenic Strains of Aspergillus flavus in Telangana and Andhra Pradesh, Int. J. Pure App. Biosci.5(6): 663-673 (2017). doi: http://dx.doi.org/10.18782/2320-7051.3091
