International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (617) Last page : (628)
Article doi: http://dx.doi.org/10.18782/2320-7051.2694

Haemato-Biochemical and Hepatic Changes in Labeo rohita Fingerlings Exposed to Multiple Stressors of Crowding and Feed Deprivation

Shamna N., Sahu N. P.*, Fawole F. J., Jain K. K. and Sarvendra K.
Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education,
Mumbai, India 400061
*Corresponding Author E-mail: npsahu@cife.edu.in

 ABSTRACT

In the present study, we observed the effect of crowding and short term (48 h) feed deprivation in Labeo rohita fingerlings. A total of 150 fingerlings (9 ± 0.5g) were distributed in three treatment groups in triplicates. Group with normal stocking density (10 no/ 75L) and normal feeding (satiation) was referred as control (C) whereas a high stocking density (20 no/ 75L) and normal feeding was referred as T1 group. A high stocking density and absolute starvation group was considered as T2 group. Blood samples were collected at regular intervals from 3h, 9h, 24 h and 48 h, and tissue samples were collected at 48 h. Higher blood glucose level was recorded at 9 and 48 h in T2 and T1 group, respectively. Nitroblue tetrazolium assay value also followed similar trend like blood glucose after 9 h. Haemoglobin level was significantly higher in T1 (6.02 ± 0.30) and T2 (6.27±0.18) group after 48h compared to control (5.27 ± 0.13) .The RBC, glutathione-s-transferase (GST) and glutamate oxaloacetate transaminase (GOT) activity in the liver showed no significant difference among the groups. Activities of hepatic superoxide dismutase (SOD), catalase, glutamate pyruvate transaminase (GPT) and glucose-6- phosphate dehydrogenase (G6PDH) followed a similar trend with the highest activity of all these enzymes wererecorded in the liver of T1 group at 48h. But, T2 group showed highest (P<0.05) lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) activities in liver while there was no significant difference (P>0.05) in liver glutathione peroxidase activity between T1 and T2 group. A significantly higher (p<0.01) hepatic glutathione and malondialdehyde concentration were observed in T1 group.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Shamna, N., Sahu, N.P., Fawole, F.J., Jain, K.K. and Sarvendra, K., Haemato-Biochemical and Hepatic Changes in Labeo rohita Fingerlings Exposed to Multiple Stressors of Crowding and Feed Deprivation, Int. J. Pure App. Biosci.5(5): 617-628 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2694