International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (40) Last page : (47)
Article doi: http://dx.doi.org/10.18782/2320-7051.4036

In vitro Regeneration in Callus Culture of Gliricidia [Gliricidia sepium (Jacq.) Steud.]

Kaushlya Choudhary, M. L. Jakhar*, Aparna, Ravi Kumar and Hari Ram Jat
Department of Plant Breeding and Genetics,
SKN College of Agriculture, Jobner (Jaipur) 303329
*Corresponding Author E-mail: mljakhar@yahoo.com

 ABSTRACT

An efficient protocol was developed for callus induction and shoot regeneration in Gliricidia [Gliricidia sepium (Jacq.) Steud.]. Callus induction was observed at majority levels of plant growth regulators, however, profuse callus induction was observed on MS medium supplemented with 0.25 and 0.5 mg/l BAP alone and in combination 0.5 mg/l (BAP) + 2.5 mg/l (IAA).The shoot morphogenesis was observed in callus when incubated at0.5 mg/l (BAP) + 2.5 mg/l (IAA), upon subculture on same levels of plant growth regulator. De novo shoot organogenesis from callus cultures were observed with 50 – 60 % frequency.Photoperiod regime 14:10 was found best for shoot bud and callus induction. Highest root induction was observed on 0.5 mg/l IAA under in vitro proliferated shoot with 100 % frequency.

Key words: Gliricidia, callus induction, regeneration, plant growth regulator.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Choudhary, K., Jakhar, M.L., Aparna, Kumar, R. and Jat, H.R., In vitro Regeneration in Callus Culture of Gliricidia [Gliricidia sepium (Jacq.) Steud.], Int. J. Pure App. Biosci.5(5): 40-47 (2017). doi: http://dx.doi.org/10.18782/2320-7051.4036