International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (380) Last page : (385)
Article doi: http://dx.doi.org/10.18782/2320-7051.3029

PCR Analysis of Genes from Aeromonas punctata for Reactive Black 5 Dye Degradation

  Roopa Kambathanalli Basavaraju and Usha Malavalli Subbaiah*
Department of Microbiology, Centre for P.G. Studies, 18/3, 9th Main, 3rd Block, Jayanagar,
Jain University, Bangaluru-11. Karnataka, India
*Corresponding Author E-mail: bg.ushams@gmail.com
Received: 26.05.2017  |  Revised: 30.06.2017   |  Accepted: 2.07.2017

 ABSTRACT

In this present investigation, the culture Aeromonas punctata was subjected to plasmid curing with 300 μg/ml of Ethidium bromide as a curing agent. This experiment demonstrated that genes responsible for degradation of Reactive black 5 dye are plasmid borne. The enzymes namely azoreductases, peroxidases, laccases and monoxygenase played a major role in the degradation of the azo dye Reactive Black 5 into nontoxic products.PCR analysis was carried out to confirm the presence of genes responsible for production of degradative enzymes by using a set of primers corresponding to the consensus sequences. The PCR amplification of laccase gene fragment was close to 800 bp, azoreductase gene fragment was close to 700 bp, peroxidase gene fragment was nearby to 600 bp and monoxygenase gene fragment lies between 500 bp and        600 bp.

Key words: Reactive Black 5 dye, Azoreductase, Peroxidase, Laccase, Monoxygenase, PCR.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Roopa,  K.B. and Usha, M.S., PCR Analysis of Genes from Aeromonas punctata for Reactive Black 5 Dye Degradation, Int. J. Pure App. Biosci.5(5): 380-385 (2017). doi: http://dx.doi.org/10.18782/2320-7051.3029