International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (348) Last page : (352)
Article doi: http://dx.doi.org/10.18782/2320-7051.5352

Phylogenetic Studies of Unculturable Halophilic Bacteria in the Fossil Sediments of Ariyalur Basin

Edward. A
Assistant Professor, Department of Biotechnology, St. Joseph’s College, Tiruchirapalli, Tamil Nadu, India
*Corresponding Author E-mail: cisgene.edward@gmail.com
Received: 30.07.2017  |  Revised: 27.08.2017   |  Accepted: 4.09.2017  

 ABSTRACT

Metagenomics is a habitat based method to study the unculturable mixed microbial populations which are difficult for cultivation based approaches. In the present investigation, an attempt was done to study the uncultivable microbial diversity from the fossil sediments of Ariyalur through 16S rRNA gene sequencing. Two uncultured bacterial 16S rRNA gene sequences were cloned in the vector respectively and subjected to 16S rRNA sequencing. The revealed two 16S rRNA gene sequences showed the high similarity to Bacillus oceanisediminis and Acinetobacter indicus. The microbial data suggested the sea invasion theory of Ariyalur.

Keywords: Metagenomics, Ariyalur, Acinetobacter indicus, Bacillus oceanisediminis, microbial diversity.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Edward, A., Phylogenetic Studies of Unculturable Halophilic Bacteria in the Fossil Sediments of Ariyalur Basin, Int. J. Pure App. Biosci.5(5): 348-352 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5352