International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (280) Last page : (286)
Article doi: http://dx.doi.org/10.18782/2320-7051.2765

Isolation and Characterization of Anti Phytopathogenic Bacteria from Finger Millet Rhizosphere

Poonam Kumari*, R. S. Netam, S. C. Yadav, A. K. Thakur and Prafull Kumar
S G College of Agriculture and Research Station, Jagdalpur, IGKV, Raipur, CG- 494001
*Corresponding Author E-mail: poonam15sep@gmail.com
Received: 28.03.2017  |  Revised: 10.04.2017   |  Accepted: 11.04.2017  

 ABSTRACT

Phytopathogenic attack is a major constraint in achieving potential yield in finger millet. Rhizobacteria present an eco-friendly and cost-effective alternatives to chemical pesticides. In order to study bacterial diversity having antagonistic potential, a total of 45 rhizobacterial isolates were isolated from 18 soil samples collected from finger millet rhizosphere. Screening for antagonism against phytopathogens revealed that 15 isolates inhibited the mycelial proliferation of Pyricularia grisae and Rhizoctonia solani in the range of 22.22-44.44% and 16.66-33.33% respectively, under dual plate assay. Cultural and morphological characterization of isolates revealed great diversity among them. Most of the isolates appeared as white, creamish white to cream colonies on nutrient agar. Isolate A-4 exhibited characteristic yellow-green pigmentation tentatively indicating its close relation with Pseudomonas sp. Biochemical characterization revealed that all were catalase producers, 13 hydrolyzed starch and showed positive result for citrate utilization. Hydrogen sulphide production was observed with only two isolate while 4 tested positive for TSIA test. Inoculating broth supplemented with different sugars revealed that glucose was utilized by 5 isolates, starch by 4, maltose by 2 and mannitol by 1 while none utilized lactose.

Keywords: Rhizobacteria, Antagonism, P. grisea, R.solani

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kumari, P., Netam, R.C., Yadav, S.C., Thakur, A.K. and Kumar, P., Isolation and Characterization of Anti Phytopathogenic Bacteria from Finger Millet Rhizosphere, Int. J. Pure App. Biosci.5(5): 280-286 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2765