International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (258) Last page : (263)
Article doi: http://dx.doi.org/10.18782/2320-7051.5308
Transcript Expression Profiling of SnRK1 (Sucrose Non-Fermenting-1-Related Protein Kinase 1) under Terminal Heat Stress in Wheat (Triticum aestivum L.)
Neeta Pargi*, S. Barthakur and Maryam Hasan
Division of Molecular Biology and Biotechnology,
NRCPB- Indian Agricultural Research Institute, New Delhi 110 012, India
*Corresponding Author E-mail: neeta.pargi@gmail.com
Received: 25.07.2017 | Revised: 7.08.2017 | Accepted: 9.08.2017
ABSTRACT
With ensuing global climate change high temperatures are becoming an important limiting factor of yield and quality and crops will have to adjust to even higher temperatures in the near future. The grain-filling stage of wheat crop is very sensitive to high temperatures. SnRK1 is a key metabolic regulator which plays an important role in plant carbon, nitrogen metabolism and development. This serine/threonine kinases family of enzyme play a central regulatory metabolism by responding to cellular energy change through relative AMP and ATP concentrations. The key role that SnRK1 play at the interface between metabolic and stress signaling makes them potential candidates for manipulation to improve crop performance in extreme environments. In the present study a transcript expression profiling was carried out in cultivar of wheat HD2967. Two vegetative stages and five reproductive-grain development stages were exposed to high temperature stress at 37ºC and individual, independent earheads were utilized for transcript profiling of wheat SnRK1 gene. Contrasting expression was observed with lower expression in early vegetative stages and higher expression for generative stages for HD2967. As a whole, these transcript expression findings show that SnRK1s are involved in diverse physiological processes throughout the life cycle of wheat plants, from the time of seedling establishment to high temperature induction to senescence.
Keywords: SnRK1, Terminal Heat Stress, Metabolic Sensor, Grain Filling Stage, Reproductive stage.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Pargi, N., Barthakur, S. and Hasan, M., Transcript Expression Profiling of SnRK1 (Sucrose Non-Fermenting-1-Related Protein Kinase 1) under Terminal Heat Stress in Wheat (Triticum aestivum L.), Int. J. Pure App. Biosci.5(5): 258-263 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5308
