International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (234) Last page : (240)
Article doi: http://dx.doi.org/10.18782/2320-7051.5469

An Attempt to Produce Oat Haploids Using Oat X Maize Hybridization Technique

Rahul Kapoor¹* and Gagandeep Singh²
Department of Plant Breeding and Genetics, PAU, Ludhiana
¹Assistant Forage Breeder, Department of Plant Breeding and Genetics, PAU, Ludhiana
²Ex-Project Fellow, Department of Plant Breeding and Genetics, PAU, Ludhiana
*Corresponding Author E-mail: rahulkapoor@pau.edu
Received: 19.08.2017  |  Revised: 30.08.2017   |  Accepted: 1.09.2017  

 ABSTRACT

The aim of the study was to develop oat haploids through crossing between oat as a female and maize as male parent followed by embryo rescue. Four oats genotypes of diverse origin along with popular fodder maize variety J-1006 were crossed at Forage Research farm, PAU, Ludhiana. Various factors influencing the growth and development of crosses like oat genotype, used as female, method of emasculation and time of pollination, type of growth regulators, their concentration and time of application were investigated. The frequency of embryo formation varied significantly between the genotypes studied. The maximum frequency was observed in genotype IC-372489 (37.50) followed by EC-528919 (14.66), IC-372415 (0.65) and IC-372510 (0.29). Embryo production is strongly influenced by the time interval between emasculation and pollination. The auxins tested have significant influence on the number of enlarged ovaries and embryos. Ovaries treated with Dicamba were generally bigger in size than the ovaries treated with 2,4-D. A higher number of embryos were obtained when oat x maize florets were treated with auxins (both 2,4-D and Dicamba) after 2 days of pollination than after 1 and 3 days.

Key words: Haploids, Oat x Maize, Pollination, Embryo rescue, Auxins

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kapoor, R. and Singh, G., An Attempt to Produce Oat Haploids Using Oat X Maize Hybridization Technique, Int. J. Pure App. Biosci.5(5): 234-240 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5469