International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (1529) Last page : (1537)
Article doi: http://dx.doi.org/10.18782/2320-7051.5133

Antioxidant Status during In vitro Plant Regeneration in Lucas aspera Spreng

Poornima D.Vijendra^1&2, Sathisha G. Jayanna¹*, Vadlapudi Kumar², Hari Gajula²,
Rajashekar J.²,Torankumar Sannabommaji² and Giridhara Basappa^2
1Department of Biochemistry, Kuvempu University, Jnana Sahyadri, Shankaraghatta - 577451 Karnataka, India
²Department of Biochemistry, Davangere University, Shivagangothri, Davangere-577002 Karnataka, India
*Corresponding Author E-mail: satishlec@rediffmail.com

 ABSTRACT

Lucas aspera Spreng. (Lamiaceae) is an important medicinal plant, belongs to lamiaceae family.   Till-date there have been no published reports on in vitro regeneration of L. aspera, hence, in the present study an attempt has been made to demonstrate the influence of antioxidant state during the in vitro plant regeneration in this plant.  Internode explants from wild grown plants were collected, surface sterilized and cultured on MS medium supplemented with different concentrations and combinations of cytokinins and auxins for the in vitro regeneration. The highest number of shoot buds were induced on MS medium supplemented with 1.5 mg/L BAP and 0.1 mgL-1 NAA combination. Rooting was induced in the individual shoot regenerants on half-strength MS medium supplemented with 0.1mgL-1 IBA. Biochemical changes during in vitro shoot regeneration was monitored for 28 days. Changes in the levels of antioxidant enzymes like ascorbate peroxidase, catalase, superoxide dismutase and glutathione reductase were monitored.  Results of the investigations confirm that the in vitro shoot regeneration event is free from the oxidative stress as the changes in antioxidant enzymes confirm the active regeneration and the plant growth regulator concentration and combination is optimal for the in vitro regeneration.

Key words: Lucas aspera, L-ascorbate, reduced glutathione, antioxidant enzymes.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Vijendra, P.D., Jayanna, S.G., Kumar, V., Gajula, H., Rajashekar, J., Sannabommaji, T. and Basappa, G., Antioxidant Status during In vitro Plant Regeneration in Lucas aspera Spreng., Int. J. Pure App. Biosci.5(5): 1529-1537 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5133