International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (1128) Last page : (1137)
Article doi: http://dx.doi.org/10.18782/2320-7051.5824
Assessment of Microbes in Liquid Tea Compost: A Qualitative Screening
Paushali Pal1a*, Nazma Khatun2b and Sudip Kumar Banerjee1c
1Techno India University, EM-4, Sector-V, Saltlake, Kolkata – 700091, West Bengal, India
2Galaxy Educare Pvt. Ltd. 2nd Floor, 197 (New no. 429), N.S.C Bose Road,
Pratapgarh E.M Bypass, Kamalgachhi More, Kolkata – 700103, West Bengal, India
*Corresponding Author E-mail: paushalipal@gmail.com
Received: 2.10.2017 | Revised: 23.10.2017 | Accepted: 25.10.2017
ABSTRACT
An attempt to screening the microbes in the liquid tea compost. The liquid tea compost prepared from the waste tea leaves under shed drying. The water added to the compost, followed by brewing, aerating and sieving. The microbes, identified by Gram’s staining, motility, biochemical test and selective media culture. In results, the shape of microorganisms viz. rods with bulged heads as positive, fluffy rods as negative, coccobacilli as negative and big rod as positively identified by Gram’s staining. For motility, yeast was higher, moderate Clostridium sp. and Azotobacter sp. and slightly motile Pseudomonas aeruginosa. In biochemical tests, methyl red, VP and indole showed negative. For starch hydrolysis and H2S utilization, Azotobacter sp. was only positive while rests were negative. Azotobacter sp. and Pseudomonas aeruginosa positive while Clostridium sp. and yeast negative for catalase. Pseudomonas aeruginosa was negative while rests were positive for glucose fermentation. During sucrose fermentation, Clostridium sp. and Azotobacter sp. were positive but Pseudomonas aeruginosa and yeast were negative. Lactose fermentation and gelatin hydrolysis, all species were positive except yeast and Azotobacter sp. and Pseudomonas aeruginosa were positive while Clostridium sp. and yeast were negative for mannitol fermentation. The Azotobacter sp. was positive while others were negative for urease and Azotobacter sp. and Pseudomonas aeruginosa were positive while Clostridium sp. and yeast were negative in citrate utilization. In conclusion, the screening of microbes in a liquid tea compost, were identified and confirmed by the above-mentioned tests. The field study is suggesting to know the efficacy of this bio-fertilizer.
Key words: Solid and liquid tea compost; Biochemistry and motility; Gram’s positive and negative; Bio-fertilizer; Green compost
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Pal, P., Khatun, N. and Banerjee, S.K., Assessment of Microbes in Liquid Tea Compost: A Qualitative Screening, Int. J. Pure App. Biosci.5(5): 1128-1137 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5824
