International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (1085) Last page : (1091)
Article doi: http://dx.doi.org/10.18782/2320-7051.5337

Extraction and Molecular Characterization of Collagen from Poultry Meat Processing by-Product (Chicken Skin)

K. Arunmozhivarman*, Robinson J. J. Abraham, V. Appa rao and M. Parthiban
Department of Livestock Products Technology (Meat Science), Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai – 600 007,
*Corresponding Author E-mail: arunmozhivet@gmail.com,
Received: 27.07.2017  |  Revised: 30.08.2017   |  Accepted: 6.09.2017  

 ABSTRACT

A study was conducted to utilize less value chicken skin, a by-product obtained during poultry meat processing to extract collagen and to determine type and level of collagen available in it. Collagen was extracted with 0.5M acetic acid containing 1% pepsin after pretreatment of samples to remove non collagenous protein and fat, using 0.1N NaOH and 20% ethanol. The extracted collagen was lyophilized and characterized. SDS-PAGE study revealed that collagen had molecular pattern with two α chain (α 1 and α 2) and one ß chain which is indicative of Type-I collagen. UV spectrum analysis of collagen was done and found that no contamination with non collagen protein. The amino acid analysis showed that glycine was the major component and less amount of lysine, isoleucine. FTIR study showed the characteristic amide band at3305.19 cm-1, 2922.52 cm-1, 1633.98 cm-1, 1549.08 cm-1,1238.07 cm-1 for amide A, B, I, II and III of collagen respectively. The micro architecture studies of collagen using Scanning Electron Microscopy confirmed that the collagen is fibrillar in structure. The result of our study clearly indicated that chicken skin has significant amount of Type-I collagen which can be extracted using acetic acid with pepsin method.

Key words: Chicken skin, by-product utilization, collagen extraction, Type I collagen, value addition.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Arunmozhivarman, K., Abraham, R.J.J., Appa rao, V. and Parthiban, M., Extraction and molecular characterization of collagen from poultry meat processing by-product (chicken skin), Int. J. Pure App. Biosci.5(5): 1085-1091 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5337