International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (1063) Last page : (1069)
Article doi: http://dx.doi.org/10.18782/2320-7051.5780
Isolation and Evaluation of Native Rhizobium Isolates for Urdbean (Vigna mungo L.) from Acid Soils
Prahalad Kumar1, D. Dash1, S. K. Joshi 2* and Yugal Kishor Sahu2
1Department of Agricultural Microbiology, College of Agriculture,
2Department of Soil Science and Agricultural Chemistry, College of Agriculture,
Indira Gandhi Krishi Vishwavidyalaya, Raipur 492012 (Chhattisgarh), India
*corresponding author e-mail: sanjaykumarjoshi784@gmail.com
Received: 24.09.2017 | Revised: 26.10.2017 | Accepted: 28.10.2017
ABSTRACT
A Greenhouse experiment was conducted under sterilized sand culture conditions at the Department of Agricultural Microbiology, IGKV, Raipur during Kharif, 2014-15 with Urdbean crop using different soil inoculums collected from various parts of Korba District of Chhattisgarh with a view to screen out low pH tolerant isolates. The pH range of these soils was 4.0 to 7.0 out of which 76 % of soil samples fall under pH 4.0-6.0. By plant infection method, 182 root nodulating bacteria obtained, out of which, a total of top 46 isolates were screened out as per evaluated by their plant assay test on the basis of growth performance like shoot biomass accumulation, nodulation no. and native soil pH Among 46 isolates, 12 isolates were identified as superior and were temperature (50°C) and acidity (5.0pH) tolerant showing nodule no. 35-21 plant -1and shoot dry matter accumulation of 1.53-1.17 g plant -1. Isolates Rhi-Ku187, Rhi-Ku34 and Rhi-Ku166 were found to be potent low pH (4.0pH) tolerant and also showed survivality at 45°C temperature. Rhi-Ku187 accumulated highest shoot dry matter of 1.533g plant -1, showing plant height of 44.90cm and nodule no. plant-1 (33). and Rhi-ku34 was found maximum no. of nodule of 35.00 plant -1. Colony characterization, survivality and growth performance of urdbean revealed that native isolate Rhi-Ku187 was considered most effective followed by Rhi-Ku 34 which would be a better choice for inoculation of of urdbean crop in an acidic soil condition of Chhattisgarh.
Key words: Urdbean, Acid tolerant, Temperature tolerant, Rhizobium, Nodulation.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Kumar, P., Dash, D., Joshi, S.K. and Sahu, Y.K., Isolation and Evaluation of Native Rhizobium Isolates for Urdbean (Vigna mungo L.) from Acid Soils, Int. J. Pure App. Biosci.5(5): 1063-1069 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5780
