International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (1023) Last page : (1029)
Article doi: http://dx.doi.org/10.18782/2320-7051.5269
IDetection of Beta-Lactam Resistance in Arcobacter Species of Animal and Human Origin
Madupuru Soma Sekhar1*, Tumati Srinivasa Rao1, Chinnam Bindu Kiranmayi 1, Kothapalli Venkata Subramanyam2 and Noorbasha Mohammad Sharif3
1Department of Veterinary Public Health and Epidemiology, 2Department of Veterinary Microbiology,
NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India
3Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, India
*Corresponding Author E-mail: somasekharmadupuru@gmail.com
Received: 21.07.2017 | Revised: 26.08.2017 | Accepted: 1.09.2017
ABSTRACT
A set of 41 Arcobacter isolates (A. butzleri, 16; A. cryaerophilus, 13; A. skirrowii, 12) isolated from diverse sources like faecal swabs of livestock (21), raw foods of animal origin (13) and human stool samples (7) were screened for beta-lactam resistance by disc diffusion method and PCR targeting blaTEM, blaSHV, blaOXA, blaAmpC, blaCTX-M group-1 and 2 beta-lactamase genes. Resistance to aztreonam (65.8%), cefotaxime (63.4%), ceftazidime (58.5%) and ceftriaxone (53.6%) was detected, with an overall frequency of 80.4% (33/41) beta-lactam resistance. Extended Spectrum Beta-lactamase (ESBL) phenotype was confirmed in a total of 15 (36.5%) Arcobacter isolates. Beta-lactamase genes were detected in 63.4% of Arcobacter isolates, with blaTEM being the predominant gene detected (51.2%, 21/41) followed by blaCTX-M group-1 (36.5%, 15/41), blaAmpC (29.2%, 12/41), blaOXA (29.2%, 12/41), blaSHV (14.6%, 6/41) and blaCTX-M group-2 (14.6%, 6/41) genes. CTX-M beta-lactamase was found to be the most frequent mechanism of ESBL resistance in Arcobacter isolates. The results highlighted the beta-lactam resistance in Arcobacter species, with special emphasis on ESBL phenotype, which is of grave concern to animal and human health in this region.
Key words: Arcobacter, beta-lactam resistance, beta-lactamase genes, ESBL.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Sekhar, M.S., Rao, T.S., Kiranmayi, C.B., Subramanyam, K.V. and Sharif, N.M., Detection of Beta-Lactam Resistance in Arcobacter Species of Animal and Human Origin, Int. J. Pure App. Biosci.5(5): 1023-1029 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5269
