International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (1013) Last page : (1022)
Article doi: http://dx.doi.org/10.18782/2320-7051.5298
In-vitro Anthelmintic and Acaricidal Activity of Nicotiana tabacum Leaf Extract Mediated AgNPs Against Rhipicephalus (Boophilus) microplus
B. Avinash1, N. Supraja2a, T.N.V.K.V. Prasad2b* and M. Alpha Raj3
1Department of Veterinary Parasitology, College of Veterinary science, Sri Venkateswara Veterinary University, Tirupathi- 517501
2Nanotechnology laboratory, Institute of Frontier Technology, Regional Agricultural Research Station, Acharya N G Ranga Agricultural University, Tirupathi- 517502, A.P., India
3Department of Veterinary Pharmacology and Toxicology, Sri Venkateswara Veterinary University, College of Veterinary Science, Proddatur-516360, Andhra Pradesh, India
*Corresponding Author E-mail: krishna.supraja@gmail.com
Received: 24.07.2017 | Revised: 29.08.2017 | Accepted: 5.09.2017
ABSTRACT
A green synthesis of silver nanoparticle was carried out using Nicotiana tabacum leaf extract. Synthesized nanoparticles were characterized using UV-Vis absorption spectroscopy, DLS, TEM, FT-IR and XRD studies respectively. UV-Vis absorption spectroscopy of prepared silver colloidal solution showed absorption maxima at 385 nm. TEM analysis showed average particle size ranges from 5nm-100nm, while XRD confirms SAED pattern confirmed the crystalline nature of synthesized nanoparticles. FT-IR analysis indicated the involvement of carboxyl (C=O), hydroxyl (O-H) and amine (N-H) functional groups of Nicotiana tabacum leaf extract in preparation of silver nanoparticles. DLS shows the particle size of silver nanoparticles is 90.5nm and zeta potential is 22.9mv. Hence from this study we concluded that the nanoparticles synthesized from Nicotiana tabacum leaf extract shown very effective on in-vitro acaricidal activity (0.170 mg/ ml to 0.0027 mg/ml of AgNPs) were used and noted cent percent mortality was at 0.170 mg/ ml and anthelmintic activity (IC50: 0.621 &IC90: 3.476 mg/ml) AgNPs of Nicotiana tabacum was found to be more lethal.
Key words: Ag nanoparticle; Acaricidal Activity; Anthelmintic Activity; Nicotiana tabacum
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Avinash, B., Supraja , N., Prasad , T.N.V.K.V. and Raj , M.A., In-vitro Anthelmintic and Acaricidal Activity of Nicotiana tabacum Leaf Extract Mediated AgNPs against Rhipicephalus (Boophilus) microplus, Int. J. Pure App. Biosci.5(5): 1013-1022 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5298
