International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (893) Last page : (910)
Article doi: http://dx.doi.org/10.18782/2320-7051.5411
Isolation of Biosurfactant Producing Organisms from the Petroleum Contaminated Soil in Gujarat
Neekita Charan1* and Shivangi Patel2
1Department of Life Science, HNGU Patan/ Hemchandracharya Nourth Gujarat University, India
2Department of Microbiology, Smt. S.M Panchal Science College Talod,/Hemchandracharya
Nourth Gujarat University, India
*Corresponding Author E-mail: charanniky@gmail.com
Received: 9.08.2017 | Revised: 16.09.2017 | Accepted: 23.09.2017
ABSTRACT
During the study of isolation of bio surfactant producing microorganism from the petroleum contaminated soil, 58 polycyclic aromatic hydrocarbons (PAH) degrading bacteria were isolated.14 isolates were screened for the biosurfactant production To check the potential of the utilization of different Hydrocarbons, the selected 5 isolates were identified and further assessed. The isolates were screened for ability to grow on petroleum fractions like petrol, diesel, kerosene, lubricant oil, paraffin oil, crude oil as sole source of carbon,on the basis of qualitative Study, 2 isolates (Ps5, Pc, two of them which were found high active to utilize crude oil and biosurfactant production. Two isolates were characterized using morphological and biochemical tests to pseudomonas spp. Two isolates showed the growths on mineral salts medium as the carbon source and energy as demonstrated by the increase in cells forming unit (CFUml) during the incubation period.The two isolates were tested to producing biosurfactant by four assays (E24 assay, Drop collapse test,Cell surface hydrophobicity, Bacterial Adhesion to Hydrocarbons (BATH test and CTAB Agar Test) these assays suggested that two isolates producing biosartactant. This study conclude the ability of pseudomonas spp. isolated from oil contaminated soil to grow on the enriched media with the hydrocarbon as a sole source of energy with high potential capacity of oil degradation and Biosurfactant production, it can be used for removal of oil from sand could enhance in situ bioremediation of soils contaminated with polycyclic aromatic hydrocarbons (PAHs)
Key word: pseudomonas spp, Petrolium, Biodegradation, Biosurfactant, isolation of PHB degrading bacteria.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Charan, N. and Patel, S., Isolation of Biosurfactant Producing Organisms from the Petroleum Contaminated Soil in Gujarat, Int. J. Pure App. Biosci.5(5): 893-910 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5411
