International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (776) Last page : (786)
Article doi: http://dx.doi.org/10.18782/2320-7051.2717
Synthesis Characterization and Evaluation of the Antimicrobial Activity of Neem Leaf Extract-Mediated Silver Nanoparticles
Avinash B.1, Supraja N.2*, Santhi Priya Ch.3, Prasad T.N.V.K.V.2 and Alpha Raj M.4
1Department of Veterinary Parasitology, Sri Venkateswara Veterinary University,
College of Veterinary Science, Tirupati-517502, A.P. India
2Nanotechnology laboratory, Acharya N. G. Ranga Agricultural University, Regional Agricultural Research Station, Tirupati-517502, A.P. India
3Rice research unit, Acharya N. G. Ranga Agricultural University, Bapatla-522101, A.P. India
4Department of Veterinary Pharmacology and Toxicology, Sri Venkateswara
Veterinary University, College of Veterinary Science, Proddatur-516 360, A.P. India
*Corresponding Author E-mail: tnvkvprasad@gmail.com
Received: 18.03.2017 | Revised: 27.04.2017 | Accepted: 8.05.2017
ABSTRACT
Now a days Production and applications of green silver nanoparticles is been an attracting research activity. In this study we report the aqueous leaf extract of Azadirachta indica was used as the reducing agent for the synthesis of silver nanoparticles (AgNPs) from silver nitrate solution. AgNPs formation was confirmed by UV-VIS, FT-IR, XRD, DLS and TEM. UV–VIS spectrum of the aqueous medium containing silver nanoparticles exhibited absorption peak at around 405 nm. Fourier transform infrared spectroscopic analysis shows the absorption peaks at 580.90, 622.83, 1050.20, 1252.08, 1394.30, 1636.89, 1772.82, 2107.52, 2821.94, 2885.18, 2989.34 and 3,361.38 cm-1. X-ray diffraction analysis conformed the face centered cubic structure of the synthesized AgNPs. By dynamic light scattering technique hydrodynamic diameter (12.4nm) and zeta potential (-34.3mV) were measured. TEM analysis revealed AgNPs were spherical in shape and were poly-dispersed. The measured average size of AgNPs was 5-50nm. Antimicrobial efficacy of synthesized AgNPs was tested (in -vitro) by disc diffusion method against both bacteria and Fungi. A green synthesized silver nanoparticle has the potent antimicrobial activity. In addition, toxicity tests were conducted to analyze the toxicological effects of particle size on Brine shrimps. Less toxicity on brine shrimps were noted after exposure to aqueous extracts and synthesized AgNPs.
Key words: Azadirachta indica, Antimicrobial efficacy, AgNPs, Nanoparticles
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Avinash, B., Supraja, N., Priya, S.Ch., Prasad, T.N.V.K.V., Alpha. Raj M., Synthesis Characterization and Evaluation of the Antimicrobial Activity of Neem Leaf Extract-Mediated Silver Nanoparticles, Int. J. Pure App. Biosci.5(5): 776-786 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2717
