International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (740) Last page : (748)
Article doi: http://dx.doi.org/10.18782/2320-7051.5620
Evaluation of Genetic Diversity Studies in Gymnema sylvestre Assessed through RAPD Markers
M. Prashanti1*, B. Arunjyothi2, K. Kalpana1 and K. Janardhan Reddy1
1Department of Botany, 2Department of Genetics,
University College of Science, Osmania University, Hyderabad, Telangana-500007
*Corresponding Author E-mail: prashantilavu@gmail.com
Received: 6.09.2017 | Revised: 20.10.2017 | Accepted: 22.10.2017
ABSTRACT
Genetic diversity studies among Gymnema sylvestre accessions were analyzed using randomly amplified polymorphic DNA (RAPD) markers. A total of twenty random decamer primers were used to analyze the diversity studies among twenty four G.sylvestre accessions which generated a total of 140 bands. Jaccard’s similarity coefficient showed a wide range (00.12 to 0.95) of variability among all twenty four accessions. UPGMA cluster analysis grouped the G.sylvestre variants into two major clusters, cluster-A and cluster-B. RAPD studies revealed that 74% genetic similarity has been recorded among the accessions collected from different regions. This marker studies will be helpful in conservation of Gymnema species.
Key words: Gymnema sylvestre, anti diabetic, genetic diversity, molecular markers, RAPD markers.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Prashanti, M., Arunjyothi, B., Kalpana, K. and Reddy, K.J., Evaluation of Genetic Diversity Studies in Gymnema sylvestre Assessed through RAPD Markers, Int. J. Pure App. Biosci.5(5): 740-748 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5620
