International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (723) Last page : (727)
Article doi: http://dx.doi.org/10.18782/2320-7051.5437

Identification of Polymorphic Loci between Donors and Recipient Parents for Marker Assisted Backcross Breeding in Rice (Oryza sativa L.)

P. J. Harikrishnan*, V. G. Jayalekshmy, Siddhesh R. N., Soumya S. L.
College of Agriculture, Vellayani, Thiruvananthapuram, Kerala, India
*Corresponding Author E-mail: hareesnanpj@gmail.com
Received: 14.08.2017  |  Revised: 25.08.2017   |  Accepted: 26.08.2017  

 ABSTRACT

The study deals with three varieties; two blast resistance gene donors PTB21 (Thekkan) and PTB7 (Parambuvattan) and recurrent parent Uma (MO16). The three parental lines were screened using the 30 chosen microsatellite loci through polymerase chain reaction (PCR) carried out in a 25 µl reaction volume comprising 50 ng template DNA, 10 pmoles of each primer, 10 mM dNTPs, 10X PCR buffer (10 mM Tris, pH 8.4, 50 mM KCl, 1.8 mM MgCl2 and 0.01 mg/ml gelatin) and 1 U of Taq DNA polymerase. Of the 30 loci, 12 were found to be polymorphic for Uma and PTB21 while 17 markers were polymorphic for Uma and PTB7. The identified polymorphic loci can be effectively used in marker assisted backcross breeding programme for the speedy recovery of recurrent parent (Uma) genome.

Key words: Molecular markers, Backcross breeding, Resistance, Genes, Blast.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Harikrishnan, P.J., Jayalekshmy, V.G., Siddhesh, R.N., Soumya, S.L., Identification of Polymorphic Loci between Donors and Recipient Parents for Marker Assisted Backcross Breeding in Rice (Oryza sativa L.), Int. J. Pure App. Biosci.5(4): 723-727 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5437