International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (698) Last page : (701)
Article doi: http://dx.doi.org/10.18782/2320-7051.3021
RT-PCR Based Diagnosis of Soybean Mosaic Virus
H. V. Nandakishor1, B. Kumaraswamy2*, S. S. Mane3 and G. Amrutha Veena4
Department of Plant Pathology, Post Graduate Institute, Dr. PDKV, Akola, 444104 Maharashtra, India
*Corresponding Author E-mail: nandakishorehv@gmail.com
Received: 25.05.2017 | Revised: 7.06.2017 | Accepted: 8.06.2017
ABSTRACT
The occurrence of Soybean mosaic virus (SMV) in soybean was confirmed by symptomatology, reverse transcription-polymerase chain reaction (RT-PCR). SMV on soybean cv. JS-335 produced characteristic symptoms such as dark green colour of leaves, mosaic and mottling symptoms, crinkling, leaf puckering of leaves. The soybean mosaic virus was detected and diagnosed by applying the RT-PCR technique. The RT- PCR technique was performed using a specific primer pair CIF/CIRev and POT1/POT2. RT- PCR technique revealed presence of SMV, with a band length of 700 bp and 725 bp have been obtained from SMV infected samples.
Key words: Soybean, Soybean mosaic virus, RT-PCR.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Nandakishor, H.V., Kumaraswamy, B., Mane, S.S. and Veena, G.A., RT-PCR Based Diagnosis of Soybean Mosaic Virus, Int. J. Pure App. Biosci.5(4): 698-701 (2017). doi: http://dx.doi.org/10.18782/2320-7051.3021
