International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (663) Last page : (670)
Article doi: http://dx.doi.org/10.18782/2320-7051.2654
SNP Identification in Exon 5 of Aquaporin 7 (AQP7) Gene and their Association with Sperm Quality Traits in Surti Bulls
Ragini Kumari1*, K. P. Ramesha2, Rakesh Kumar3, P. Divya4, Beena Sinha1 and
Jyanendr Kumar Shahi5
1Ph.D. Scholar, Animal Genetics and Breeding Division, ICAR- National Dairy Research Institute,
Karnal (Haryana) - 132001, India
2Principal Scientist, Dairy Production Section, SRS of ICAR- National Dairy Research Institute,
Bengaluru (Karnataka) - 560030, India
3Assistant Animal Scientist, Regional Research Station (Punjab Agricultural University),
Ballowal Saunkhari (Punjab) - 144521, India
4Research Program Coordinator, Central Research Station, BAIF, Pune (Maharashtra) - 412202, India
5Teaching Associate, Department of Livestock Products Technology, R.V.C, B.A.U.,
Kanke Ranchi, Jharkhand – 834006 India
*Corresponding Author E-mail: raginindri@gmail.com
Received: 3.03.2017 | Revised: 12.03.2017 | Accepted: 14.03.2017
ABSTRACT
The aim of the present study was to study genetic polymorphism in exon 5 of AQP7 gene and to analyze their association with semen quality traits in Surti bulls (n=21). Blood samples were collected from 21 Surti bulls maintained at Centralized Semen Collection Centre, Dharwad, Karnataka and genomic DNA was isolated using high salt method. The quantity of DNA was assessed in a spectrophotometer and quality by gel electrophoresis. The amplicon size of 287 bp covering exon 5 region of AQP7 gene was amplified by polymerase chain reaction (PCR) and the products were subjected to single strand conformation polymorphism (SSCP) analysis. Four unique SSCP banding patterns were revealed by subsequent silver staining of the polyacrylamide gels. The presence of single nucleotide polymorphisms (SNPs) was confirmed by direct sequencing of the PCR products. Sequence analysis revealed 7 SNPs (4 in exonic and 3 in intronic region) as compared to Bos taurus (Ensemble Ref Seq: ENSBTAG00000020105). The statistical analysis using repeated general linear model procedure (SAS 9.3) for association study indicated that exon 5 SSCP variants were significantly associated (P≤0.01) with per cent live sperm in Surti bulls. Bulls with pattern 3 had lowest mean viability and differed significantly from other three patterns. These results suggest that there exists a considerable genetic variation at exon 5 region of AQP7 gene and there is possibility of using this as candidate gene for genetic improvement of buffaloes for sperm quality.
Key words: AQP7 gene, Surti bulls, PCR-SSCP, SNP, Repeated measures of GLM
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Kumari, R., Ramesha, K.P., Kumar, R., Divya, P., Sinha, B. and Shahi, J.K., SNP Identification in Exon 5 of Aquaporin 7 (AQP7) Gene and their Association with Sperm Quality Traits in Surti Bulls, Int. J. Pure App. Biosci.5(4): 663-670 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2654
