International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (647) Last page : (650)
Article doi: http://dx.doi.org/10.18782/2320-7051.5222
Screening of Lines for Restoring Fertility Genes Rf3 and Rf4 with SSR Markers in Rice (Oryza sativa L.)
K. Rajendra Prasad1*, K. V. Radha Krishna2, S. Sudheer Kumar3, P. Senguttuvel4 and
L. V. Subba Rao5
1Ph.D Scholar, Department of Genetics and Plant Breeding, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad-500030, Telangana, India
2Deputy Director of Research, PJTSAU, Rajendranagar, Hyderabad-500030, Telangana, India
3Registrar, PJTSAU, Rajendranagar, Hyderabad-500030, Telangana, India
4Scientist, Hybrid Rice Section, Indian Institute of Rice Research, Rajendranagar,
Hyderabad-500030, Telangana, India
5Principal Scientist & Head, Crop Improvement Section, Indian Institute of Rice Research,
Rajendranagar, Hyderabad-500030, Telangana, India
*Corresponding Author E-mail: krprasad456@gmail.com
Received: 3.08.2017 | Revised: 8.08.2017 | Accepted: 9.08.2017
ABSTRACT
Tostudy the identification of restorers with different molecular markers which are linked to fertility restorer genes Rf3 and Rf4 of WA-CMS system, seventy two parental lines with no information on fertility restoration were screened with the help of molecular markers linked to major fertility genes Rf3 and Rf4. The SSR primer RM6100 linked to Rf4 gene on chromosome 10 and RM10313 linked to Rf3 gene on chromosome 1 are used efficiently for identifying restorer lines in crop improvement programmes. Therefore, these markers are useful tool for evaluating large number of breeding lines to know about their fertility restoration in a short period of time without generating and evaluating large number of test crosses. The potential restorers may be identified with hundred percentage efficiency based on molecular screening itself, if candidate genes based markers are developed and validated for both Rf4 and Rf3 genes.
Key words: Fertilityrestoration,Rf4,Rf3,molecularmarkers, WA-CMS
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Prasad, K.R., Krishna, K.V.R., Kumar, S.S., Senguttuvel, P. and Rao, L.V.S., Screening of Lines for Restoring Fertility Genes Rf3 and Rf4 with SSR Markers in Rice (Oryza sativa L.), Int. J. Pure App. Biosci.5(4): 647-650 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5522
