International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (550) Last page : (560)
Article doi: http://dx.doi.org/10.18782/2320-7051.3002
RAPD-PCR Finger Printing of Brinjal Germplasm Against Fruit Rot of Brinjal
Sanjeev P. Jakatimath1*, R. K. Mesta1, P. S. Ajjappalavar2, Sadanand K. Mushrif3 and
I. B. Biradhar4
1Department of Plant Pathology (University of Horticultural Sciences) Bagalkot-587104, Karnataka, India
2Horticultural Research Station (University of Horticultural Sciences) Devihosur-581110,
Haveri, Karnataka, India
3Department of Plant Pathology (University of Horticultural Sciences) Kolar-563101, Karnataka, India
4Department of Agronomy (University of Horticultural Sciences), Arabavi-591307, Karnataka, India. India
*Corresponding Author E-mail: jakatimathsanjeev7@gmail.com
Received: 22.05.2017 | Revised: 4.06.2017 | Accepted: 5.06.2017
ABSTRACT
A total of 60 genotypes screened under filed condition against fruit rot of brinjal. Among 22 genotypes which showed different categories of resistant reaction were selected for the molecular characterization that helped in the reliable varietal selection programme for resistance breeding. All genotypes were analyzed by molecular parameters. For molecular characterization 34 RAPD markers were used for screening. Only 25 RAPD decamer primers were selected for the genetic analysis of all representative genotypes. The extent of genetic diversity and construction of phylogenetic tree was done by NTYSES software. Genetic similarity varied from 0.01 to 0.93 from each of the primer analyzed. Among the twenty two RAPD screened genotypes few showed the polymorphism. The whole phylogeny tree falls in two clusters namely C1 & C2 using Jaccard similarity coefficient of 0.01. The cluster C2 again divided into different sub clusters namely SCA and SCB. Among all the genotypes represented in the phylollogenic tree, line-3 (K12d1012-6) & line-11 (K12D1052-1) were farthest to each other; however line-17 (K12D1036-1) & line-21(K12D1011-5) were very closely related. Even though line 3 and line 19 belongs to different clusters with less co-efficient they look alike with respect to resistance against the fruit rot.
Key words: Eggplant, Resistance, Fruit rot, Characterization, RAPD.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Jakatimath, S.P., Mesta, R.K., Ajjappalavar, P.S., Mushrif, S.K. and Biradhar, I.B., RAPD-PCR Finger Printing of Brinjal Germplasm Against Fruit Rot of Brinjal, Int. J. Pure App. Biosci.5(4): 550-560 (2017). doi: http://dx.doi.org/10.18782/2320-7051.3002
