International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (516) Last page : (525)
Article doi: http://dx.doi.org/10.18782/2320-7051.4028
Optimization of Phytohormone Concentrations for Callus Induction in Mature Embryos of Finger Millet (Eleucine coracana)
Mahendra Kumar Sharma1, Anamika Kashyap1, B. R. Singh1, Rajesh Kumar Singhal1* and Jyoti Chauhan2
1Department of Molecular Biology and Genetic Engineering,Pantnagar, (Uttrakhand), 263145
2Deparment of Plant Physiology, Institute of Agricultural Sciences, BHU, Varanasi, (U.P) 221005
*Corresponding Author E-mail: rajasinghal151@gmail.com
Received: 10.06.2017 | Revised: 19.06.2017 | Accepted: 21.06.2017
ABSTRACT
Finger millet (Eleusine coracana (L.) Gaertn.) is the primary food source for millions of people in tropical dry land regions of the world. Compared to other millet, finger millet grains also have a relatively higher content of minerals such as calcium, phosphorus, iron and manganese. It has nutritional qualities superior to that of rice and is on par with that of wheat. Finger millet is vulnerable to biotic stresses like fungal blast and abiotic stresses like drought and salinity stress.
Development of efficient tissue regeneration system is an essential prerequisite for successful production of transgenic plants. For callus induction and plant regeneration in finger millet GE-3885, mature embryos were cultured onto Murashige and Skoog (MS) medium supplemented with different concentrations of 2, 4-D alone and in combination with BAP NAA and Kinetin. Callus induction was observed in all 42 different combination of phytohormone. Callus initiation was observed in 16 different combinations. Results showed that the highest callus was induced observed in MS medium containing 1.5 mg/lNAA.
Key words: 2,4-D (2,4- Di Chloro Phenoxy Acetic Acid). BAP (Benzyl Amino Purine), NAA (Nepthelic Acetic Acid), M.S Media, Callus Induction.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Sharma, M.K., Kashyap, A., Singh, B.R., Singhal, R.K. and Chauhan, J., Optimization of Phytohormone Concentrations for Callus Induction in Mature Embryos of Finger Millet (Eleucine coracana), Int. J. Pure App. Biosci.5(4): 516-525 (2017). doi: http://dx.doi.org/10.18782/2320-7051.4028
