International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (37) Last page : (50)
Article doi: http://dx.doi.org/10.18782/2320-7051.5416
Screening and Characterization of Biosurfactant Producing Bacteria Found in Mar Chika Lagoon
Ikram Kamal1*, Fatima Azzahra Lahlou1,2, Mohamed Blaghen1, Lee Kangmin3 and Abderrahmane Hammoumi1
1Laboratory of Microbiology, Pharmacology, Biotechnology and Environment
Faculty of Sciences Ain-Chock, route El Jadida, B.P. 5366, Casablanca, Morocco
2Mohammed VI University of Health Sciences (UM6SS), Faculty of Medicine, National Reference Laboratory. Anfa City, Boulevard Taïeb Naciri, Commune Hay HassaniCasablanca 20000, Morocco
3 Technology Lab.Dept. Molecular Biology Chonbuk Natl. University
*Corresponding Author E-mail: kamal.ikram@gmail.com
Received: 9.08.2017 | Revised: 18.08.2017 | Accepted: 20.08.2017
ABSTRACT
Petroleum based products are the major source of energy for industries and daily life. Leaks and accidental spills occur regularly during the exploration, production, refining, transport, and storage of petroleum and petroleum products. In the present study four Bacterial strain isolated from Marchika lagoon, located in the north-west Mediterranean coast of Morocco were identified by biochemical and molecular tests. The effects of culture conditions in vitro and biosurfactant detection were studied on bacterial strains capable of degrading gasoline. Biosurfactant production was evaluated by measuring the hydrophobicity of the cell surface, the hemolytic activity, the spreading technique of Oil spreading, drop-collapse, and surface tension. Therefore a chemical identification of produced biosurfactants was investigated using Fourier Transform Infrared (IR-TR) and High-performance liquid chromatography Electrospray Ionization Mass Spectra methods. Aeromonas salmonicida seems to be the most potent biosurfactant producing showing a β-hemolysis with higher hydrophobicity index (BATH) and degree of collapse and the lowest surface tension. The optimal growth conditions including pH, temperature and the concentration of gasoline were respectively determined at 7, 25°C and 1%. HPLC and IF showed the presence of some biosurfactants and a large variety of compounds showing same properties as biosurfactants.
Key words: Biosurfactant, Aeromonas salmonicida, Marchika lagoon, Bioremediation.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Kamal, I., Lahlou, F.A., Blaghen, M., Kangmin, L. and Hammoumi, A., Screening and Characterization of Biosurfactant Producing Bacteria Found in Mar Chika Lagoon, Int. J. Pure App. Biosci.5(4): 37-50 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5416
