International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (2147) Last page : (2157)
Article doi: http://dx.doi.org/10.18782/2320-7051.5307
Biochemical Characterization of Ralstonia solanacerum Causing Bacterial Wilt of Brinjal in The Hilly District of Assam
Madhusmita Kataky*, Ajit Kr. Tamuli1, Robindra Teron1 and Ramen Kr. Sarma2
*Krishi Vigyan Kendra, Kamrup
1Department of Life Science & Bioinformatics, Assam University, Assam
2Assam Agricultural University, Jorhat, Assam
*Corresponding Author E-mail: madhusmitakataky@gmail.com
Received: 26.07.2017 | Revised: 29.08.2017 | Accepted: 3.09.2017
ABSTRACT
Ralstonia solanacearum causes bacterial wilt of solanaceous crop plants including brinjal, a most devastating disease in humid tropic. A survey was conducted to study the the status of bacterial wilt incidence in major brinjal growing areas and to characterize the isolates of R. solanacearum causing bacterial wilt of brinjal in the Hilly District Karbi Anglong of Assam. The survey results reveals that the incidence of bacterial wilt disease on Brinjal varied from its lowest 24.28 per cent at Rongkhoi area in Hamren subdivision to the highest 51.87 percent at Taralangso in Diphu subdivision. Among the three different sub division the intensity of damage caused by the bacterial wilt disease of Brinjal was found the highest in Diphu Sub Division (36.54%) followed by Bokajan Sub-Division (34.01%).
The isolates of R. solanacearum were obtained from different locations.Gram’s staining and Potassium hydroxide solubility test revealed that all groups of R. solanacearum isolates are gram negative. The isolates of R. solanacearum fermented four basic sugars (Dextrose, sucrose, manitol and lactose). These results of all biochemical tests in combination with the pathogenicity test confirmed the isolates were R. solanacearum causing bacterial wilt of brinjal. All groups of R. Solanacearum isolates were found virulent producing pink or light red color or characteristic red center and whitish margin on TZC medium after 24 hours of incubation. On the biovar test clearly revealed that all groups of R. solanacearum isolates oxidized disaccharides (Sucrose, lactose, and maltose) and sugar alcohols (manitol, sorbitol and dulcitol) within 3-5days and confirmed biovar as III. Pathogencity test on tomato and chilli indicating wide host range of R. solanacearum isolates and categorized them in race 1. Therefore, it may be confirmed that R. solanacearum causing bacterial wilt of brinjal in Hilly District of Assam belong to Biovar III and Race 1.
Key words: Bacterial wilt, Brinjal, Characterization, Ralstonia solanacerum, Karbi, Anglong
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Kataky, M., Tamuli, A.K., Teron, R. and Sarma, R.K., Biochemical Characterization of Ralstonia solanacerum Causing Bacterial Wilt of Brinjal in The Hilly District of Assam, Int. J. Pure App. Biosci.5(4): 2147-2157 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5307
