International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (1908) Last page : (1912)
Article doi: http://dx.doi.org/10.18782/2320-7051.5764
Studies on Transmission of Cucumber Mosaic Virus (CMV) by Sap Inoculation in Tomato
P. Jalender1*, Bharati N Bhat 2, K. Anitha3 and K. Vijayalakshmi4
1&2Department of Plant Pathology, PJTSAU, Rajendranagar, Hyderabad (500 030) India
3National Bureau of Plant Genetic Resources (NBPGR), Rajendranagar, Hyderabad (500 030) India
4National Institute of Plant Health Management, Rajendranagar, Hyderabad (500 030) India
*Corresponding Author E-mail: porikajalender089@gmail.com
Received: 12.07.2017 | Revised: 25.07.2017 | Accepted: 26.07.2017
ABSTRACT
Tomato plants were observed free from local lesions on the inoculated leaves. However, systemic infection was noticed on the 8th day after inoculation. Electron micrographs obtained with leaf dip preparation revealed the presence of spherical particles of 28.5 nm diameter indicating the presence of CMV belonging to Cucumo virus group. DAC-ELISA results revealed that of the 31 genotypes, including Arka Vikas, all were found positive to CMV, except EC620389, which was also symptomless. All symptomatic genotypes responded positively for CMV by DAC-ELISA. Though the genotypes, EC251790 and EC625642 did not exhibit any characteristic symptoms of CMV even after second inoculation, reacted positively against CMV antiserum during DAC-ELISA.
Key words:Kharif, Systemic Infection, Virus, Tomato.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Jalender , P., Bhat , B.N., K. Anitha , and Vijayalakshmi K., Studies on Transmission of Cucumber Mosaic Virus (CMV) by Sap Inoculation in Tomato, Int. J. Pure App. Biosci.5(4): 1908-1912 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5764
