International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (1416) Last page : (1423)
Article doi: http://dx.doi.org/10.18782/2320-7051.5213

Enzymatic Study of Glutamine Synthetase Activity for Validating Nitrogen Use Efficiency in Rice Genotypes

K. Rajesh*, Ramesh Thatikunta and D. Saida Naik
Department of Crop Physiology, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad-500030, Telangana, India
*Corresponding Author E-mail: kunta.rajesh3@gmail.com
Received: 14.07.2017  |  Revised: 25.07.2017   |  Accepted: 26.07.2017  

 ABSTRACT

The field experiment was laid out in a split plot design with two nitrogen levels of 60 and 120 kg N ha-1 as main plot treatments and twenty six rice genotypes as sub plot treatments and was replicated thrice during 2011and 2012. Glutamine synthetase (GS) is one of the selection criteria to identify nitrogen use efficient cultivars and their use in developing mapping population for high nitrogen use efficiency (NUE). GS activity was more in plants growing at low nitrogen levels than that at high nitrogen levels in rice. Maximum glutamine synthetase activity was recorded in the genotype BPT-5204 when 60 kg N ha-1 (0.94 mmol g-1 min-1) was applied at maturity stage as compared to application of 120 kg N ha-1. MTU-1001 recorded maximum NUE in 60 kg N ha-1 (83.68) and minimum was recorded in 120 kg N ha-1 (45.53). The results showed that the NUE did not increase linearly with the amount of nitrogen application and higher nitrogen levels showed significantly lower NUE values.

Key words: Rice, Nitrogen, Glutamine synthetase enzyme and Nitrogen use efficiency

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Rajesh, K., Thatikunta, R. and Naik, D.S., Enzymatic Study of Glutamine Synthetase Activity for Validating Nitrogen Use Efficiency in Rice Genotypes, Int. J. Pure App. Biosci.5(4): 1416-1423 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5213