International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (1240) Last page : (1246)
Article doi: http://dx.doi.org/10.18782/2320-7051.4072
Effects of Irradiated Carriers, Storage Temperatures, on Rhizobium Bioinoculant at Different Intervals
G. Thirumal*, R. Subhash Reddy, S. Triveni and M. V. Bhave
Department of Agricultural Microbiology and Bioenergy,
College of Agriculture, Prof Jaya Shankar Telangana State Agricultural University, Rajendranagar,
Hyderabad - 500 030, Telangana, India
*Corresponding Author E-mail: thirumalgummula18@gmail.com
Received: 16.06.2017 | Revised: 24.06.2017 | Accepted: 25.06.2017
ABSTRACT
The production and quality of rhizobial inoculants in many developing countries is limited by the availability of suitable carriers or technological limitations. Experiments were conducted to evaluate the potential of various inexpensive and widely available carrier materials. The carriers, evaluated, were Vermicompost, Vermiculate, Lignite, and Sodium Alginate, the study contrasted on two effective factors carrier materials and storage temperatures, a total of ten Rhizobial bacterial isolates collected from different sources. These cultures were studied morphologically and biochemically for purity confirmation then screened for PGPR properties, among all Rhizobial isolates, GNR-1 showed best plant growth promoting abilities in in-vitro conditions. The selected efficient PGP Rhizobium (GNR-1) and isolate was multiplied in large quantities in appropriate culture broth by incubating at 28±2°C in an incubator shaker at 120 rpm till they attained log phase with a cell load of l×l09cfu ml-1. The carriers sterilized by gamma irradiation at a dose rate of 5.0 kGy for 1h then carriers were used for bioinoculant preparation and stored at different temperatures i.e. 4ºC and 28ºC. The bacterial population highest in 1st month log10 value 9.76 in sodium alginate carrier based biofertilizer stored at 40C and at 8th month the survival rate of rhizobial cells was very highest in sodium alginate log10 8.94 stored at 4ºC less in lignite log10 value 5.50 stored at 28±2°C.
Key words: Rhizobium, carriers, irradiated sterilization, different temperatures, sodium alginateFull Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Thirumal, G., Reddy, R.S., Triveni, S. and Bhave, M.V., Effects of Irradiated Carriers, Storage Temperatures, on Rhizobium Bioinoculant at Different Intervals , Int. J. Pure App. Biosci.5(4): xxx-xxx (2017). doi: http://dx.doi.org/10.18782/2320-7051.4072
