International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (1189) Last page : (1196)
Article doi: http://dx.doi.org/10.18782/2320-7051.5624
Screening of Bhut Jolokia (Capsicum chinense Jacq.) Germplasm of North East India against Chilli Leaf Curl virus
Prashanth Kumar Adluri*, Gajendra Mohan Baldoldiya and Palash Deb Nath
Department of Plant Pathology, Assam Agricultural University, Jorhat 785013
*Corresponding Author E-mail: adluriprashanth@gmail.com
Received: 2.08.2017 | Revised: 10.08.2017 | Accepted: 11.08.2017
ABSTRACT
Bhut Jolokia (Capsicum chinense Jacq.) is a type of chilli extensively grown in North Eastern region of India, predominantly in the states of Assam, Nagaland and Manipur. Bhut Jolokia is recognized as the 7th hottest chilli in the world having a scoville heat unit of 1,041,427. Leaf curl virus disease of chilli is considered as the most serious disease problem prevailing in Bhut Jolokia growing North Eastern region causing serious losses.The disease is characterized by severe upward curling, reduction in leaf size, leaf thickening, vein clearing and stunted plant growth. The severely affected plants are stunted bearing hardly any fruits. PCR detection technique was standardised using two chilli leaf curl virus specific primers at various annealing temperatures using gradient PCR. Primer pairs ChLCVF1-ChLCVR1 and ChLCVF2-ChLCVR2 successfully yielded 550bp and 568bp PCR products at annealing temperature of 45 °C and 48.9 °C, respectively.Thirty different Bhut Jolokia genotypes were collected from various localities of Assam, Nagaland and Manipur based on their fruit morphological characters. These genotypes were screened against ChLCV under field conditions and confirmed by PCR detection using ChLCVF2-ChLCVR2 primer pair at 180 days after transplanting, resulted 4 highly susceptible, 11 susceptible, 14 moderately susceptible and 1 symptomless genotypes. Partial sequencing of three ChLCV isolates showed 99.00 per cent similarity. However, sequence similarity search of these isolates with 20 known ChLCV isolates worldwide recorded 86.00 per cent to 97.00 per cent similarity. Sequence similarity of Bhut Jolokia ChLCV isolates showed 87.00 per cent to 90.00 per cent homology with Indian ChLCV isolates indicating the virus from Jorhat to be distinct strain from Indian isolates, for which the name ChLCV-Bhut Jolokia Jorhat (ChLCV-BJ-JRT) strain is proposed.
Key words: Bhut Jolokia, PCR, ChLCV (Chilli leaf curl virus), Scoville heat units, Partial sequencing
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Adluri, P.K., Baldoldiya, G.M. and Nath, P.D., Screening of Bhut Jolokia (Capsicum chinense Jacq.) Germplasm of North East India against Chilli Leaf Curl virus, Int. J. Pure App. Biosci.5(4): 1189-1196 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5624
