International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (916) Last page : (919)
Article doi: http://dx.doi.org/10.18782/2320-7051.5566
Applications of CRISPR-Cas system in Plant Pathology: A Review
Afsha Tabassum1, Shweta2 and Vishnutej Ellur3*
1&3Ph.D. student, Department of Plant Pathology, Washington State University, Pullman, Washington, USA
2Ph.D. student, Dept. of Genetics & Plant breeding, University of Agricultural Sciences,
Raichur, Karnataka, India – 584104
*Corresponding Author E-mail: afshatabassum1@gmail.com
Received: 8.08.2017 | Revised: 16.08.2017 | Accepted: 17.08.2017
ABSTRACT
Gene editing of living organisms has been tired by scientists since the discovery of DNA. Earlier approaches of gene editing was using oligonucleotides, self-splicing introns etc. Zinc Finger Nucleases (ZFN’s) and TALENs are effective but not widely adapted. The CRISPR (Clustered Regularly Interspaced Palindromic Repeat) – Cas (CRISPR- associated) is hundreds of millions of years old found in bacteria as survival mechanism against infection of viruses. The mechanism of CRISPR-Cas was understood only in early 2000s. This review presents overview of CRISPR technology and its applications against Plant Pathogens including Fungi, Bacteria and Viruses.
Key words: Gene editing, Zinc Finger Nucleases, TALENs, CRISPR-Cas, Plant Pathogens
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Tabassum, A., Shweta and Ellur, V., Applications of CRISPR-Cas system in Plant Pathology: A Review, Int. J. Pure App. Biosci.5(4): 916-919 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5566
