International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (818) Last page : (824)
Article doi: http://dx.doi.org/10.18782/2320-7051.5518
Efficacy of Biocontrol Agents against Seed Mycoflora of Sunflower at Different Storage Periods
Srinivas, A.*, Pushpavathi, B., Lakshmi, B. K. M. and Shashibushan, V.
Department of Plant Pathology, College of Agriculture,
Professor Jayashankar Telangana State Agricultural University, Hyderabad-500030
*Corresponding Author E-mail: sunny.srinivas76@gmail.com
Received: 31.07.2017 | Revised: 7.08.2017 | Accepted: 8.08.2017
ABSTRACT
The efficacy of three bioagents viz., Trichoderma viride, Bacillus subtilis, Pseudomonas fluorescens tested both alone and in combinations @ 10 g kg-1 seed against seed mycoflora of sunflower and at different storage periods (upto 3 months) were studied.A total of 16 seedborne fungi belonging to 13 genera viz., Alternaria sp., Macrophomina phaseolina, Aspergillus flavus, Aspergillus niger, Aspergillus ochraceus, Aspergillus ustus, Emericella nidulans, Fusarium sp., Epicoccum sp., Cladosporium sp., Curvularia sp., Chaetomium sp., Drechslera sp., Rhizopus sp., Trichoderma sp. and Penicillium sp. were recovered from untreated and treated seeds at different storage periods. Among the biocontrol agents, Trichoderma viride (20.66%) was found significantly superior to other biocontrol agents in inhibiting the seed mycoflora followed by Pseudomonas fluorescens + Trichoderma viride (23.59%) and the least effective (60.66%) was Pseudomonas fluorescens. The per cent seed infection by different seed mycoflora increased with the increase in storage period. However, there was a gradual decline in field mycoflora viz., Alternaria sp., Macrophomina phaseolina, Fusarium sp. and Drechslera sp. and gradual increase in storage mycoflora viz., Aspergillus flavus, Aspergillus niger, Cladosporium sp., Curvularia sp. etc. was found with the increase in storage period.
Key words: Sunflower seed mycoflora, Biocontrol seed treatments, storage mycoflora, standard blotter method
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Srinivas, A., Pushpavathi, B., Lakshmi, B.K.M. and Shashibushan, V., Efficacy of Biocontrol Agents against Seed Mycoflora of Sunflower at Different Storage Periods, Int. J. Pure App. Biosci.5(4): 818-824 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5518
